2019 Fiscal Year Final Research Report
Plasma Biochemistry and Metabolic Profiles of Cells Interacted with Non-thermal Plasmas
| Project/Area Number |
17H02805
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plasma electronics
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| Research Institution | Kyushu University (2019)
Nagoya University (2017-2018) |
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Principal Investigator |
Ishikawa Kenji 九州大学, プラズマナノ界面工学センター, 特任教授 (60417384)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | 低温プラズマ / 非平衡大気圧プラズマ / メタボローム / 細胞内代謝 / 活性酸素窒素種 / グリオーマ / プラズマ活性有機物 / 乳酸 |
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| Outline of Final Research Achievements |
The alternation of intracellular metabolites cultured in plasma-activated solutions was observed in brain tumor cells (U251SPs). Bioenergetics and biomass production were taken place through catabolism from glucose uptake in the cell culture medium; (i) bioenergetics were deficient in the plasma-activated culture medium due to reduced activity of glycolytic enzymes due to oxidative stress; (ii) lactate-containing solutions enhanced anabolism from lactate uptake while maintaining a reduced intracellular state; and (iii) plasma-activated organics acted as inhibitors of glutamine assimilation and lipid metabolism in the plasma-activated lactate solution (PAL). In particular, we found that intracellular metabolism was altered by plasma-activated organic matter in PAL, leading to plasma-induced cell death.
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| Free Research Field |
プラズマバイオ
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| Academic Significance and Societal Importance of the Research Achievements |
大気圧下でガス温度の上昇を伴わない非平衡（低温）プラズマの生成が制御されて可能となり，液体や生体をはじめとする様々な対象にプラズマの照射効果が見いだされていた中，本研究は，プラズマ活性溶液中で培養された細胞内のメタボローム解析を世界で初めて実施した，研究開始当初はプラズマが生成するRONSの影響が生体作用の主要な因子であるといった見解から，溶液中の有機物の変性をプラズマが液体に作用した結果に生じており、その溶液中に産生された成分が細胞の恒常的応答を変動させ、代謝産物における明瞭な違いを世界で初めて明らかにした．
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