Development of new diagnostic method for refractory maliganant diseases

2018 Fiscal Year Annual Research Report

• Project/Area Number: 17H04110
• Research Institution: Kyoto University
• Principal Investigator: 足立 壯一 京都大学, 医学研究科, 教授 (10273450)
• Co-Investigator(Kenkyū-buntansha): 上久保 靖彦 京都大学, 医学研究科, 特定教授 (60548527)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: ゲノム解析 / フローサイトメトリー法 / レーザーマイクロダイセクション / 癌遺伝子 / 予後因子

Outline of Annual Research Achievements

『難治性造血器悪性腫瘍・固形腫瘍における新規共通増殖機構の解明とそれをターゲットとした新規検査法の開発』を深化・実用化するために(1)～(2)を通じた研究・開発を行う。
(1)難治性腫瘍の新規予後因子の探索とそれを利用した新規検査法の開発
A:急性骨髄性白血病の新規予後因子探索；AML-05登録患者検体を用いて、網羅的遺伝子解析を遂行中であり、t(8;21)及びMLL転座型AMLにおいて、新規遺伝子変異を見出し、変異遺伝子の機能解析を遂行中である。t(8;21)細胞株を用いて、in vitro及びin vivoにおける細胞死誘導機序を解析し、CDK4/6阻害剤によるin vitro及びin vivoにおける抗腫瘍効果を検討する。B: 難治性腫瘍予後不良因子をターゲットとした新規遺伝子スイッチ法の開発 ；CML、MLL-AML細胞株を用いたPI-Pライブラリー（SAHA、Chb-conjugated）スクリーニングを施行し、IC50が1μM以下のHITsを取得する。野生型マウスを使用したHITｓの簡易毒性試験（10mg/kg・週1回投与）を実施し、毒性の低いHITｓのValidationを実施する。
(2)新規検査技術の開発　A：Xeno-Graft Banking新規マウス生体内ヒト腫瘍細胞増幅技術；JCCG 再発ALL観察研究登録中の患者検体をNOGマウスを用いて増幅、保存中である。B:フローサイトメーターによる白血病微小残存病変の検出；JCCG AML臨床試験（AML-D11）の患者検体を用いて、三重大学とともに、微小残存病変を解析し、MRD陽性例は予後不良であることを見出し、今年のアメリカ血液学会で発表予定である。C:レーザーマイクロダイセクション法の開発；JCCG再発AML観察研究登録患者の再発時検体のG-CSFR typeIV発現量解析を中央診断施設として解析中である。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

1．急性骨髄性白血病の新規予後因子探索；AML-05余剰検体を用いた網羅的遺伝子解析を、順調に遂行している。また、再発AML観察研究も開始しており、登録症例の検体も用いて解析中である。単なる新規予後因子探索に留まらず、機能解析をin vitro及びin vivoで遂行し、t(8;21)細胞株におけるCDK4/6阻害剤投与で抗腫瘍効果を得た。
2.難治性腫瘍予後不良因子をターゲットとした新規遺伝子スイッチ法の開発；PI-Pライブラリースクリーニングでは、IC50が1μM以下の複数個のHITsを取得した。野生型マウスを使用したHITｓの簡易毒性試験では、毒性の低いHITｓが存在し、現在Apoptosis Assay、Apotosis ArrayなどValidationを実施中である。
3.Xeno-Graft Banking新規マウス生体内ヒト腫瘍細胞増幅技術;JCCG 再発ALL観察研究登録患者検体を用いたNOGマウスでの増幅を順調に遂行中であり、増幅前の検体と増幅後検体の網羅的遺伝子解析を予定している。
4.フローサイトメーターによる白血病微小残存病変の検出;JPLSG AML12第3相臨床試験、JCCG初の造血細胞移植試験(AML-SCT15)登録例の移植前のFCM MRDを順調に遂行中である。また、今年度中には、ML-DS臨床試験（AML-D16）のFCM MRD解析を開始する。FCM MRD検出精度の向上に寄与するマーカー（CD56等）や、ML-DS初発時検体における白血病幹細胞マーカー（TIM-3等）の推移も解析し、MRDマーカーとしての有用性を検討する。
5．レーザーマイクロダイセクション（LMD）法の開発;登録開始となったJCCG再発AML観察研究登録検体から、白血病細胞のみを抽出し、G-CSFR typeIV発現量をJCCG中央診断施設として解析している。

Strategy for Future Research Activity

1．急性骨髄性白血病の新規予後因子探索;AML-05余剰検体を用いた網羅的遺伝子解析を継続するとともに、新規予後因子探索に留まらず、機能解析をin vitro及びin vivoで遂行する。既存の抗がん剤のみならず、スーパーコンピューターを用いた新規薬剤の開発も目指す。
2．難治性腫瘍予後不良因子をターゲットとした新規遺伝子スイッチ法の開発;HITsの6塩基配列より、ターゲット転写因子Xを同定するために、MLL-AML細胞(MV4-11)へのHIT投与後のMicroarrayを施行し、最も変動の大きなPathway抽出を行い、転写因子Xを確定する。転写因子XのConditional KD、O/Eにてその評価を行う。
3．Xeno-Graft Banking新規マウス生体内ヒト腫瘍細胞増幅技術;JCCG 再発ALL観察研究登録患者検体を用いたNOGマウスでの増幅を順調に遂行中であり、増幅前の検体と増幅後検体の網羅的遺伝子解析終了後は、新規開発された薬剤の薬効効果も検討予定である。
4．フローサイトメーターによる白血病微小残存病変の検出;JPLSG AML12第3相臨床試験、JCCG初の造血細胞移植試験(AML-SCT15)登録例の解析を継続するとともに、平成30年度中には、ML-DS臨床試験（AML-D16）のFCM MRD解析を開始する。FCM MRD検出精度の向上に寄与する新たなマーカーの有用性を検討する。
5．レーザーマイクロダイセクション（LMD）法の開発;すでに既知の遺伝子変異解析が終了しているAML-05保存細胞を用いて、LMD法を用いて、白血病細胞のみを抽出し、新たな遺伝子変異が同定できるかどうかを検討する。同様の検討は、再発AML観察研究や、AML-12登録症例においても遂行し、初発時から予後不良因子が同定できれば、小児AML患者の予後改善に貢献できることが期待できる。

Research Products

• [Journal Article] Clinical and biological features of paediatric acute myeloid leukaemia ( AML ) with primary induction failure in the Japanese Paediatric Leukaemia/Lymphoma Study Group AML ‐05 study (2019)
  • Author(s): Miyamura Takako、Moritake Hiroshi、Nakayama Hideki、Tanaka Shiro、Tomizawa Daisuke、Shiba Norio、Saito Akiko M.、Tawa Akio、Shimada Akira、Iwamoto Shotaro、Hayashi Yasuhide、Koike Takashi、Horibe Keizo、Manabe Atsushi、Mizutani Shuki、Taga Takashi、Adachi Souichi
  • Journal Title: British Journal of Haematology Volume: 185 Pages: 284～288
  • DOI: 10.1111/bjh.15799
  • Peer Reviewed
• [Journal Article] Oocyte cryopreservation for a female cancer patient after reaching adulthood (2019)
  • Author(s): Ogata Hideto、Umeda Katsutsugu、Tani Hirohiko、Horie Akihito、Adachi Souchi
  • Journal Title: Pediatrics International Volume: 61 Pages: 207～208
  • DOI: 10.1111/ped.13752
  • Peer Reviewed
• [Journal Article] Impact of low-dose irradiation and in vivo T-cell depletion on hematopoietic stem cell transplantation for non-malignant diseases using fludarabine-based reduced-intensity conditioning (2019)
  • Author(s): Katsutsugu K、、Yabe H、Kato K、Adachi S、Atsuta Y、Morio T et al.
  • Journal Title: Bone Marrow Transplantation Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1038/s41409-018-0418-8
  • Peer Reviewed
• [Journal Article] Pathogenic mutations identified by a multimodality approach in 117 Japanese Fanconi anemia patients (2019)
  • Author(s): Mori M, Hira A, Yoshida K, Muramatsu H, Okuno Y, Shiraishi Y, Anmae M, Yasuda J, Tadaka S, Kinoshita K, Osumi T, Noguchi Y, Adachi S, Kobayashi R, Kawabata H, Imai K, Morio T, Tamura K, Takaori-Kondo A, Yamamoto M, Miyano S, Kojima S, Ito E, Ogawa S, Matsuo K, Yabe H, Yabe M, Takata M.
  • Journal Title: Haematologica Volume: 印刷中 Pages: 印刷中
  • DOI: 10.3324/haematol.2018.207241.
  • Peer Reviewed
• [Journal Article] Prophylaxis and treatment with mycophenolate mofetil in children with graft-versus-host disease undergoing allogeneic hematopoietic stem cell transplantation: a nationwide survey in Japan. (2019)
  • Author(s): Kawashima N, Iida M, Suzuki R, Fukuda T, Atsuta Y, Hashii Y, Inoue M, Kobayashi M, Yabe H, Okada K, Adachi S, Yuza Y, Kawa K, Kato K
  • Journal Title: International Journal of Hematology Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1007/s12185-019-02601-5.
  • Peer Reviewed
• [Journal Article] RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells (2018)
  • Author(s): Mitsuda Yoshihide、Morita Ken、Kashiwazaki Gengo、Taniguchi Junichi、Bando Toshikazu、Obara Moeka、Hirata Masahiro、Kataoka Tatsuki R.、Muto Manabu、Kaneda Yasufumi、Nakahata Tatsutoshi、Liu Pu Paul、Adachi Souichi、Sugiyama Hiroshi、Kamikubo Yasuhiko
  • Journal Title: Scientific Reports Volume: 8 Pages: 6423
  • DOI: 10.1038/s41598-018-24969-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] RUNX1 mutations in pediatric acute myeloid leukemia are associated with distinct genetic features and an inferior prognosis. (2018)
  • Author(s): Yamato G, Shiba N, Yoshida K, Hara Y, Shiraishi Y, Ohki K, Okubo J, Park MJ, Sotomatsu M, Arakawa H, Kiyokawa N, Tomizawa D, Adachi S, Taga T, Horibe K, Miyano S, Ogawa S, Hayashi Y.
  • Journal Title: Blood. Volume: 131 Pages: 2266-2270
  • DOI: 10.1182/blood-2017-11-814442.
  • Peer Reviewed
• [Journal Article] Risk-stratified therapy for children with FLT3-ITD-positive acute myeloid leukemia: results from the JPLSG AML-05 study (2018)
  • Author(s): Akira A, Daisuke T、Takashi T, Saito AM、Keizo H、Hayashi Y、Tanaka S、Adachi S et al.
  • Journal Title: International Journal of Hematology Volume: 107 Pages: 586～595
  • DOI: 10.1007/s12185-017-2395-x
  • Peer Reviewed
• [Journal Article] High incidence of BK virus-associated hemorrhagic cystitis in children after second or third allogeneic hematopoietic stem cell transplantation. (2018)
  • Author(s): Umeda K, Kato I, Kawaguchi K, Tasaka K, Kamitori T, Ogata H, Mikami T, Hiramatsu H, Saito R, Ogawa O, Takahashi T, Adachi S.
  • Journal Title: Pediatr Transplant Volume: 22 Pages: e13183
  • DOI: 10.1111/petr.13183.
  • Peer Reviewed
• [Journal Article] Allogeneic hematopoietic stem cell transplantation for children and adolescents with high-risk cytogenetic AML: distinctly poor outcomes of FUS-ERG-positive cases (2018)
  • Author(s): Tomizawa Daisuke、Yoshida Masanori、Kondo Tadakazu、Miyamura Takako、Taga Takashi、Adachi Souichi、Koh Katsuyoshi、Noguchi Maiko、Kakuda Harumi、Watanabe Kenichiro、Cho Yuko、Fukuda Takahiro、Kato Motohiro、Shiba Norio、Goto Hiroaki、Okada Keiko、Inoue Masami、Hashii Yoshiko、Atsuta Yoshiko、Ishida Hiroyuki
  • Journal Title: Bone Marrow Transplantation Volume: 54 Pages: 393～401
  • DOI: 10.1038/s41409-018-0273-7
  • Peer Reviewed
• [Journal Article] Evaluation of high-dose cytarabine in induction therapy for children with de novo acute myeloid leukemia: a study protocol of the Japan Children's Cancer Group Multi-Center Seamless Phase II-III Randomized Trial (JPLSG AML-12). (2018)
  • Author(s): Tomizawa D, Tanaka S, Hasegawa D, Iwamoto S, Hiramatsu H, Kiyokawa N, Miyachi H, Horibe K, Saito AM, Taga T, Adachi S.
  • Journal Title: Jpn J Clin Oncol. Volume: 48 Pages: 587-593
  • DOI: 10.1093/jjco/hyy061.
  • Peer Reviewed
• [Journal Article] Progressive Restrictive Ventilatory Impairment in Idiopathic Diffuse Pulmonary Ossification. (2018)
  • Author(s): Matsuo H, Handa T, Tsuchiya M, Kubo T, Yoshizawa A, Nakayama Y, Shiga S, Hitomi T, Adachi S, Date H, Hirai T, Ichiyama S
  • Journal Title: Intern Med. Volume: 57 Pages: 1631-1636
  • DOI: 10.2169/internalmedicine.9433-17.
  • Peer Reviewed
• [Journal Article] Influence of post-transplant mucosal-associated invariant T cell recovery on the development of acute graft-versus-host disease in allogeneic bone marrow transplantation. (2018)
  • Author(s): Kawaguchi K, Umeda K, Hiejima E, Iwai A, Mikami M, Nodomi S, Saida S, Kato I, Hiramatsu H, Yasumi T, Nishikomori R, Kondo T, Takaori-Kondo A, Heike T, Adachi S.
  • Journal Title: Int J Hematol. Volume: 108 Pages: 66-75
  • DOI: 10.1007/s12185-018-2442-2.
  • Peer Reviewed
• [Journal Article] Nationwide survey of therapy-related leukemia in childhood in Japan (2018)
  • Author(s): Imamura Toshihiko、On behalf of the Leukemia/Lymphoma Committee、Taga Takashi、Takagi Masatoshi、Kawasaki Hirohide、Koh Katsuyoshi、Taki Tomohiko、Adachi Souichi、Manabe Atsushi、Ishida Yasushi、Japanese Society of Pediatric Hematology Oncology (JSPHO)
  • Journal Title: International Journal of Hematology Volume: 108 Pages: 91～97
  • DOI: 10.1007/s12185-018-2439-x
  • Peer Reviewed
• [Journal Article] High-dose chemotherapy with autologous stem cell transplantation spares re-irradiation for recurrent intracranial germinoma (2018)
  • Author(s): Kubota Hirohito、Umeda Katsutsugu、Kagehiro Kouzuki、Tanaka Kuniaki、Daifu Tomoo、Hamabata Takayuki、Nodomi Seishiro、Kato Itaru、Hiramatsu Hidefumi、Arakawa Yoshiki、Mizowaki Takashi、Adachi Souichi
  • Journal Title: Pediatric Blood & Cancer Volume: 65 Pages: e27104～e27104
  • DOI: 10.1002/pbc.27104
  • Peer Reviewed
• [Journal Article] Secondary bone/soft tissue sarcoma in childhood cancer survivors: a nationwide hospital-based case-series study in Japan (2018)
  • Author(s): Ishida Yasushi、Maeda Miho、Adachi Souichi、Rikiishi Takeshi、Sato Maho、Kawaguchi Hiroshi、Manabe Atsushi、Tokuyama Mika、Hori Hiroki、Okamura Jun、Ogawa Atsushi、Goto Hiroaki、Kobayashi Ryoji、Yoshinaga Shinji、Fujimoto Junichiro、Kuroda Tatsuo
  • Journal Title: Japanese Journal of Clinical Oncology Volume: 48 Pages: 806～814
  • DOI: 10.1093/jjco/hyy102
  • Peer Reviewed
• [Journal Article] Chronic myeloid leukemia following treatment for bilateral retinoblastoma (2018)
  • Author(s): Kamitori Tatsuya、Umeda Katsutsugu、Tasaka Keiji、Ogata Hideto、Mikami Takashi、Kato Itaru、Hiramatsu Hidefumi、Kondo Tadakazu、Adachi Souichi
  • Journal Title: Pediatric Blood & Cancer Volume: 65 Pages: e27107～e27107
  • DOI: 10.1002/pbc.27107
  • Peer Reviewed
• [Journal Article] Clinical characteristics of pediatric patients with myeloid sarcoma without bone marrow involvement in Japan. (2018)
  • Author(s): Taga T, Imamura T, Nakashima K, Maeda N, Watanabe A, Miyajima Y, Sakaguchi S, Sano H, Hasegawa D, Kawasaki H, Adachi S, Takagi M, Koh K, Manabe A, Taki T, Ishida Y
  • Journal Title: International Journal of Hematology Volume: 108 Pages: 438-442
  • DOI: 10.1007/s12185-018-2492-5.
  • Peer Reviewed
• [Journal Article] Sudden spinal hemorrhage in a pediatric case with total body irradiation-induced cavernous hemangioma (2018)
  • Author(s): Mikami T, Kato I, Nozaki F, Umeda K, Kamitori T, Tasaka K, Ogata H, Hiramatsu H, Arakawa Y, Adachi S
  • Journal Title: Pediatr Blood Cancer. Volume: 65 Pages: e27250
  • DOI: 10.1002/pbc.27250.
  • Peer Reviewed
• [Journal Article] Cytomegalovirus infection in pediatric patients with hepatoblastoma after liver transplantation. (2018)
  • Author(s): Nodomi S, Umeda K, Kato I, Saida S, Hiramatsu H, Ogawa E, Yoshizawa A, Okamoto S, Okajima H, Uemoto S, Adachi S.
  • Journal Title: Pediatr Transplant. Volume: 22 Pages: e13273.
  • DOI: 10.1111/petr.13273.
  • Peer Reviewed
• [Journal Article] .Recurrent CCND3 mutations in MLL-rearranged acute myeloid leukemia. (2018)
  • Author(s): Matsuo H, Yoshida K, Kamikubo Y, Ogawa S, Adachi S. et al
  • Journal Title: Blood Adv Volume: 2 Pages: 2879-2889.
  • DOI: 10.1182/bloodadvances.2018019398.
  • Peer Reviewed / Open Access
• [Journal Article] Sudden Intracranial Hemorrhage in a Patient With Atypical Chronic Myeloid Leukemia in Chronic Phase (2018)
  • Author(s): Kouzuki Kagehiro、Umeda Katsutsugu、Saida Satoshi、Kato Itaru、Hiramatsu Hidefumi、Funaki Takeshi、Kanda Kenji、Muramatsu Hideki、Yoshida Kenichi、Ogawa Seishi、Adachi Souichi
  • Journal Title: Journal of Pediatric Hematology/Oncology Volume: 40 Pages: e553～e556
  • DOI: 10.1097/MPH.0000000000001061
  • Peer Reviewed
• [Journal Article] Secondary cancer after a childhood cancer diagnosis: viewpoints considering primary cancer. (2018)
  • Author(s): Ishida Y, Maeda M, Adachi S, Inada H, Kawaguchi H, Hori H, Ogawa A, Kudo K, Kiyotani C, Shichino H, Rikiishi T, Kobayashi R, Sato M, Okamura J, Goto H, Manabe A, Yoshinaga S, Qiu D, Fujimoto J, Kuroda T.
  • Journal Title: Int J Clin Oncol Volume: 23 Pages: 1178-1188
  • DOI: 10.1007/s10147-018-1303-6.
  • Peer Reviewed
• [Journal Article] Sequential use of second-generation tyrosine kinase inhibitors following imatinib therapy in pediatric chronic myeloid leukemia: A report from the Japanese Pediatric Leukemia/Lymphoma Study Group (2018)
  • Author(s): Kurosawa Hidemitsu、Tanizawa Akihiko、Muramatsu Hideki、Tono Chikako、Watanabe Akihiro、Shima Haruko、Ito Masaki、Yuza Yuki、Hamamoto Kazuko、Hotta Noriko、Okada Masahiko、Saito Akiko Moriya、Manabe Atsushi、Mizutani Shuki、Adachi Souichi、Horibe Keizo、Ishii Eiichi、Shimada Hiroyuki
  • Journal Title: Pediatric Blood & Cancer Volume: 65 Pages: e27368～e27368
  • DOI: 10.1002/pbc.27368
  • Peer Reviewed
• [Journal Article] Paraneoplastic hypereosinophilic syndrome associated with IL3-IgH positive acute lymphoblastic leukemia. (2018)
  • Author(s): Kobayashi K, Mizuta S, Yamane N, Ueno H, Yoshida K, Kato I, Umeda K, Hiramatsu H, Suehiro M, Maihara T, Usami I, Shiraishi Y, Chiba K, Miyano S, Adachi S, Ogawa S, Kiyokawa N, Heike T.
  • Journal Title: Pediatr Blood Cancer Volume: 66 Pages: e27449
  • DOI: 10.1002/pbc.27449.
  • Peer Reviewed
• [Presentation] Negative CD19 Expression Is Associated with Inferior Relapse-Free Survival in RUNX1-RUNX1T1-Positive Acute Myeloid Leukemia; The Japanese Pediatric Leukemia/Lymphoma Study Group Experience from the AML-05 Study. (2018)
  • Author(s): Imamura T, Sakamoto K, Shiba N, Deguchi T, Kiyokawa N, Hashii Y, Saito AM, Tomizawa D, Taga T and Adachi S.
  • Organizer: 第60回アメリカ血液学会
  • Int'l Joint Research
• [Presentation] Clinical Feature and Genetic Alterations in Myeloid/Natural Killer (NK) Cell Precursor Acute Leukemia and Myeloid/NK Cell Acute Leukemia、 (2018)
  • Author(s): Nishimura A, Yokoyama K, Adachi S, Koh K, Manabe A, Taki T, Ishida Y, Tojo A, Takagi M. et al.
  • Organizer: 第60回アメリカ血液学会
  • Int'l Joint Research
• [Presentation] Recurrent Genomic Aberrations of D-Type Cyclins Are Therapeutic Targets of CDK4/6 Inhibitors in t(8;21) and MLL-Rearranged Acute Myeloid Leukemia、 (2018)
  • Author(s): Matsuo H, Yoshida K, Kamikubo Y, Ogawa S, Adachi S et al.
  • Organizer: 第60回アメリカ血液学会
  • Int'l Joint Research
• [Presentation] Comprehensive Analysis of 343 Genes Using Targeted Sequencing Panel By Next-Generation Sequencer in 77 Pediatric AML Patients with Normal and Complex Karyotypes: JCCG Study, JPLSG AML-05. (2018)
  • Author(s): Kaburagi T, Yamato G, Shiba N, Yoshida K, Hara Y, Shiraishi Y, Ohki K, Sotomatsu M, Arakawa H, Matsuo H, Shimada A, Kiyokawa N, Tomizawa D, Taga T, Horibe K, Miyano S, Ogawa S, Adachi S, Hayashi Y,
  • Organizer: 第60回アメリカ血液学会
  • Int'l Joint Research
• [Presentation] Effect of Age on the Prognosis of Molecular Abnormalities in Pediatric Acute Myeloid Leukemia、 (2018)
  • Author(s): Hara Y, Shiba N, Yamato G, Ohki K, Sotomatsu M, Tabuchi K, Tomizawa D, Taki T, Kinoshita A, Kiyokawa N, Arakawa H, Shibuya M, Taga T, Tawa A, Horibe K, Adachi S, Hayashi Y.
  • Organizer: 第60回アメリカ血液学会
  • Int'l Joint Research
• [Presentation] Hematopoietic Cell Transplantation for Children with Acute Megakaryoblastic Leukemia without Down Syndrome、 (2018)
  • Author(s): Hama A, Taga T, Tomizawa D, Muramatsu H, Hasegawa D, Adachi S, Yoshida N, Noguchi M, Inoue M, Okada K, Koh K, Atsuta Y, Ishida H.
  • Organizer: 第60回アメリカ血液学会
  • Int'l Joint Research
• [Presentation] Outcome of allogeneic hematopoietic stem cell transplantation for children and adolescents with acute myeloid leukemia and high-risk cytogenetics. (2018)
  • Author(s): Tomizawa D, Yoshida M, Kondo T, Miyamura T, Taga T, Adachi S, Koh K, Noguchi M, Kakuda H, Shiba N, Watanabe K, Inoue M, Hashii Y, Atsuta Y, Ishida H:
  • Organizer: 第50回国際小児がん学会
  • Int'l Joint Research
• [Presentation] Age and NRAS mutation status are possible prognostic markers in pediatric acute myeloid leukemia with CBFB-MYH11 fusion gene. (2018)
  • Author(s): Hara Y, Shiba N, Ohki K, Yamato G, Sotomatsu M, Tomizawa D, Taki T, Kinoshita A, Kiyokawa N, Arakawa H, Tawa A, Horibe K, Taga T, Adachi S, Hayashi Y
  • Organizer: 第50回国際小児がん学会
  • Int'l Joint Research
• [Presentation] Clinical significance of mutations of RAS pathway related genes including PTPN11, RAS, and CBL genes in pediatric AML -the JCCG study, JPLSG AML-05-. (2018)
  • Author(s): Kaburagi T, Yamato G, Shiba N, Hara Y, Ohki K, Sotomatsu M, Arakawa H, Kiyokawa N, Tomizawa D, Adachi S, Taga T, Horibe K, Hayashi Y.
  • Organizer: 第50回国際小児がん学会
  • Int'l Joint Research
• [Presentation] Prognostic impact of neutropenia period in patients with acute myeloid leukemia (AML) enrolled in the Japanese Pediatric Leukemia/Lymphoma Study Group AML-05 study. (2018)
  • Author(s): Aoki T, Takahashi H, Tanaka S, Shiba N, Hasegawa D, Iwamoto S, Terui K, Moritake H, Nakayama H, Shimada A, Koh K, Goto H, Kosaka Y, Saito AM, Horibe K, Kinoshita A, Tomizawa D, Tawa A, Taga T, Adachi S:
  • Organizer: 第50回国際小児がん学会
  • Int'l Joint Research
• [Presentation] Prognosis of pediatric AML patients with FLT3-ITD is predicted by DNA methylation pattern -the JCCG study JPLAG AML-05-. (2018)
  • Author(s): Yamato G, Kawai T, Shiba N, Ohki K, Hara Y, Kiyokawa N, Tomizawa D, Shimada A, Sotomatsu M, Arakawa H, Adachi S, Taga T, Horibe K, Hata K, Hayashi Y:
  • Organizer: 第50回国際小児がん学会
  • Int'l Joint Research
• [Presentation] Secondary cancer after a childhood cancer diagnosis: Viewpoints considering primary cancer. (2018)
  • Author(s): Ishida Y, Maeda M, Adachi S et al.
  • Organizer: 第50回国際小児がん学会
  • Int'l Joint Research
• [Presentation] Characteristics and outcome of children with acute myeloid leukemia and Down syndrome ineligible for clinical studies, (2018)
  • Author(s): Nakashima K, Hasegawa D, Miyamura T, Hama A, Iwamoto S, Terui K, Tomizawa D, Adachi S, Taga T
  • Organizer: 第11回小児白血病リンパ腫シンポジウム
  • Int'l Joint Research
• [Presentation] Novel Anti-Leukemia Therapeutic Strategy Through CROX(Cluster Regulation of RUNX Family) (2018)
  • Author(s): Furuichi K, Noguchi Y, Suzuki Y, Iwai S, Shimada S, Iwai A, Sugiyama H, Adachi S, Kamikubo Y:
  • Organizer: 第9回日本血液学会国際シンポジウム
  • Int'l Joint Research
• [Presentation] Highly Efficient Elimination of p210-,p190-BCR=ABL Protein Through CROX Strategy. (2018)
  • Author(s): Shimada S, Noguchi Y, Suzuki Y, Iwai S, Furuichi K, Sugiyama H, Kamikubo Y, Adachi S:
  • Organizer: 第9回日本血液学会国際シンポジウム
  • Int'l Joint Research
• [Presentation] Vascular Niche Regulation Through RUNX1 Attenuating E-Selectin. (2018)
  • Author(s): Noguchi Y, Suzuki Y, Furuichi K, Iwai S, Shimada S, Sugiyama H, Adachi S, Kamikubo Y
  • Organizer: 第9回日本血液学会国際シンポジウム
  • Int'l Joint Research
• [Presentation] Hematopoietic cell transplantation for children with acute megakaryoblastic leukemia. (2018)
  • Author(s): Hama A, Taga T, Tomizawa D, Muramatsu H, Hasegawa D, Adachi S, Kato K, Noguchi M, Inoue M, Okada K, Koh K, Atsuta Y, Ishida H:
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] The detection of minor clones with somatic KIT D816V mutations in pediatric de novo AML. (2018)
  • Author(s): Sasaki K, Shiba N, Uchiyama Y, Ikeda J, Yoshitomi M, Shimosato Y, Tokumasu M, Matsuo H, Yoshida K, Ohki K, Yamato G, Hara Y, Kinoshita A, Tomizawa D, Taga T, Adachi S, Tawa A, Horibe K, Matsumoto N, Ito S, Hayashi Y
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] Identification of recurrent CCND3 mutations in MLL-rearranged acute myeloid leukemia. (2018)
  • Author(s): Matsuo H, Yoshida K, Kamikubo Y, Ogawa S, Adachi S et al.
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] Clinical significance and prognostic analyses of PTPN11, RAS, and CBL mutations in pediatric AML. (2018)
  • Author(s): Kaburagi T, Yamato G, Shiba N, Hara Y, Ohki K, Sotomatsu M, Arakawa H, Kiyokawa N, Tomizawa D, Adachi S, Taga T, Horibe K, Hayashi Y
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] Correlation between obesity and outcome in patients with AML enrolled in the JPLSG AML-05 study. (2018)
  • Author(s): Sano H, Fukushima K, Yano M, Osone S, Kato Y, Shinkoda Y, Mori N, Ishida Y, Saito Y, Sawada A, Toyoda H, Hasegawa D, Miyamura T, Iwamoto S, Takahashi H, Terui K, Koh K, Goto H, Kosaka Y, Saito AM, Horibe K, Kinoshita A, Tomizawa D, Tawa A, Taga T, Adachi S,
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] CROX(Cluster regulation of RUNX)induces apoptoic cell death through regulatin g Gene X in APL cells. (2018)
  • Author(s): Furuichi K, Iwai A, Mikami M, Takasaki S, Obara M, Hattori E, Noguchi Y, Sugiyama H, Adachi S, Kamikubo Y:
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] Vital roke of RUNX1 both in the regulation of BCR-ABL expression and in the maintenanceof CML cells. (2018)
  • Author(s): Shimada S, Maeda S, Noguch Y, Iwai S, Suzuki Y, Sugiyama H, Kamikubo Y, Adachi S
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] Secondary bone/soft tissue sarcoma in childhood cancer survivors: A nationwide hospital based case-series study in Japan (2018)
  • Author(s): Ishida Y, Maeda M, Adachi S et al.
  • Organizer: 第56回日本癌治療学会学術集会
• [Presentation] Suppression of malignant rhabdoid tumors through novel drug based on Gene Switch Technology. (2018)
  • Author(s): Masamitsu Mikami, Tomoo Daifu, Takuya Kanatani, Kana Furuichi, Saho Takasaki, Atsushi Iwai, Yuki Noguchi, Yuta Suzuki, Etsuko Httori, Yasuzumi Matsui, Hiroshi Sugiyama, Yasuhiko Kamikubo, Souichi Adachi
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] Inhibition of BCR-ABL expression through CROX(Cluster regulation of RUNX). (2018)
  • Author(s): Sae Shimada, Yuki Noguchi, Shiina Iwai, Yuta Suzuki, Hiroshi Sugiyama, Yasuhiko Kamikubo, Souichi Adachi
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] Cluster regulation of RUNX induces apoptotic cell death through regulating gene X in acute promyelocytic leukemia (APL). (2018)
  • Author(s): Kana Furuichi, Atsushi Iwai, Masamitsu Mikami, Saho Takasaki, Moeka Obara, Etsuko Hattori, Yuki Noguchi, Toshiya Tatsuta, Yuta Suzuki, Hiroshi Sugiyama, Souichi Adachi, Yasuhiko Kamikubo:
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] Biological significance of nascent BCR-ABL revealed by modeling translocation (9;22)using CRISPR/Cas9system. (2018)
  • Author(s): Tsukimi Shoji, Asumi Yokota, Atsushi Sato, Naoka Kamio, Takahiro Kashiwagi, Yusuke Torikoshi, Yasuo Miura, Souichi Adachi, Taira Maekawa, Hideyo Hirai:
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] Combinatorial Histone Acetyltransferases(HATs) inhibition as a potential therapeutic approach for pancreatic cancer. (2018)
  • Author(s): Shino Kobyaashi, Yuki Noguchi, Erika Okinak, Natsuki Wariishi, Shiina Iwai, Sae Shimada, Yuta Suzuki, Mai Oyama, Souichi Adachi, Yasuhiko Kamikubo
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] Genetic regulation of RUNX2-cancer stem cell marker X axis in CRPC-NE cells. (2018)
  • Author(s): Yuki Noguchi, Natsuki Wariishi, Shiina Iwai, Sae Shimada, Yuta Suzuki, Mai Ohyama, Yasuzumi Matsui, Hiroshi Sugiyama, Souichi Adachi, Yasuhiko Kamikubo
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] Regulation of HAT induces apoptotic cell death through regulating hypoxia mechanism in RCC and osteosarcoma cells. (2018)
  • Author(s): Saho Takasaki, Masamitsu Mikami, Kana Furuichi, Atsushi Iwai, Moeka Obara, Yuki Noguchi, Takuya Kanatani, Yuta Suzuki, Yasuhiko Kamikubo, Souichi Adachi.
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] Negative CD19 expression is associated with inferior relapse free survival in RUNX1-RUNX1T1 positive AML; JPLSG experience from the AML-05 study. (2018)
  • Author(s): Sakamoto K, Shiba N, Deguchi T, Kiyokawa N, Hashii Y, Saito A, Tomizawa D, Taga T, Adachi S, Imamura T
  • Organizer: 第60回日本小児血液・がん学会学術集会
• [Presentation] 小児がん経験者の２次性成人型がんの解析：本邦における後方視的検討 (2018)
  • Author(s): Maho Sato, Shinji Yoshinaga, Miho Maeda, Souichi Adachi, Hiroko Inada, Hiroki Hori, Atsushi Ogawa, Ryoji Kobayashi, Hiroaki Goto, Atsushi Manabe, Kika Tokuyama, Junichiro Fujimoto, Tatsuo Kuroda, Yasushi Ishida
  • Organizer: 第60回日本小児血液・がん学会学術集会
• [Presentation] 遺伝子スイッチ法を用いた、悪性ラブドイド腫瘍に対する新規治療戦略の構築 (2018)
  • Author(s): Masamitsu Mikami, Tomoo Daifu, Takuya Kanatani, Kana Furuichi, Saho Takasaki, Atsushi Iwai, Katsutsugu Umeda, Hiroshi Sugiyama, Yasuhiko Kamikubo, Souichi Adachi
  • Organizer: 第60回日本小児血液・がん学会学術集会
• [Presentation] 神経芽細胞におけるRUNX1-TRIM24/BCL11A-p53 axisの重要性に関する検討 (2018)
  • Author(s): Yuki Noguchi, Akifumi Nakatani, Shiina Iwai, Yuta Suzuki, Sae Shimada, Masamitsu Mikami, Katsutsugu Umeda, Hiroshi Sugiyama, Souichi Adachi, Yasuhiko Kamikubo
  • Organizer: 第60回日本小児血液・がん学会学術集会
• [Presentation] 肝芽腫に対するCD146をターゲットとした新規治療の開発 (2018)
  • Author(s): Mari Sonoda, Katsutsugu Umeda, Seishiro Nodomi, Satoshi Obu, Satoshi Saida, Itaru Kato, Hidefumi Hiramatsu, Eri Ogawa, Shinya Okamoto, Ken MOrita, Yasuhiko Kamikubo, Souichi Adachi, Tatsutoshi Nakahata, Hideaki Okajima, Shinji Uemoto, Junko Takita,
  • Organizer: 第60回日本小児血液・がん学会学術集会
• [Presentation] 児悪性腫瘍患者の終末期における苦痛緩和のための持続的鎮静 (2018)
  • Author(s): Sayaka Maeda, Itaru Kato, Katsutsugu Umeda, Hidefumi Hiramatsu, Souichi Adachi, Junko Takita, Satoru Tsuneto
  • Organizer: 第60回日本小児血液・がん学会学術集会
• [Presentation] 中枢神経浸潤白血病異種移植マウスモデルにおけるpiggyBac CD19 CAR-T細胞の脳室内投与の安全性と有効性の検討 (2018)
  • Author(s): 田中邦昭、加藤 格、田中美幸、盛田大介、高橋義行、梅田雄嗣、平松英文、中畑龍俊、足立壮一、滝田順子
  • Organizer: 第60回日本小児血液・がん学会学術集
• [Remarks]
  • URL: https://adachilab-kyotouniv.amebaownd.com/
• [Remarks]
  • URL: http://www.med.kyoto-u.ac.jp/organization-staff/research/human_health/mt0302/