2019 Fiscal Year Final Research Report
Model construction for molecular regulatory mechanisms in condensation of mesenchymal stem cells during organogenesis
| Project/Area Number |
17H04415
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
吉崎 恵悟 九州大学, 歯学研究院, 助教 (10507982)
寺尾 文恵 九州大学, 歯学研究院, 助教 (10510018)
鈴木 治 東北大学, 歯学研究科, 教授 (60374948)
星 健治 九州大学, 大学病院, 助教 (90569964)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | 間葉細胞 / 細胞凝集 / 細胞遊走 / 器官発生 |
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| Outline of Final Research Achievements |
Cell condensation is a critical role for the organ morphology. However, the mechanisms are still unclear. In this study, we explored specific transcriptional start sites (TSS) of tooth organ by CAGE (Cap Analysis of Gene Expression) analysis. We identified a tooth specific TSS, which has been detected on chromosome 15qD1 region. This TSS codes microRNA-875 (mir875). MiR875-5p was specifically expressed in dental mesenchymal cells at bell stage of tooth germ.We identified that the cell migration toward to dental epithelial cells was significantly induced by miR875-5p via PDGF signaling pathway.
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| Free Research Field |
歯科矯正学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究課題は、発生期の器官形成に見られる、細胞凝集に焦点を当てて研究を行った。歯をモデルとした網羅的トランスクリプトーム解析を通して、間葉細胞凝集時に認められる遺伝子群を同定し、そのなかでもnon-coding RNAであるmiR875に着目し、研究を行った。器官発生において、細胞間の情報交換は厳密な器官形成を成し遂げる上で重要な要素であり、間葉細胞の凝集、それに続く上皮細胞への情報伝達における制御機構の一端を解明できたと考えており、今後の器官再生法への応用が期待できる。
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