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2021 Fiscal Year Annual Research Report

拍動する心筋細胞シートを用いた伸縮性多点電極アレイによる薬物反応の評価

Research Project

Project/Area Number 17H06149
Research InstitutionThe University of Tokyo

Principal Investigator

染谷 隆夫  東京大学, 大学院工学系研究科(工学部), 教授 (90292755)

Co-Investigator(Kenkyū-buntansha) 関野 正樹  東京大学, 大学院工学系研究科(工学部), 教授 (20401036)
横田 知之  東京大学, 大学院工学系研究科(工学部), 准教授 (30723481)
福田 憲二郎  国立研究開発法人理化学研究所, 開拓研究本部, 専任研究員 (40613766)
Project Period (FY) 2017-05-31 – 2022-03-31
Keywordsバイオエレクトロニクス / 伸縮性センサ / 生体適合性 / 心筋細胞 / 細胞シート / ナノメッシュセンサ / 有機電気化学トランジスタ / センサシステム
Outline of Annual Research Achievements

本研究では、創薬における心臓への副作用を定量的に評価することを目的として、自由に拍動する心筋細胞シートの活動電位を定量的に計測する手法の確立する研究を進めてきた。実際にナノメッシュセンサを用いることで、ダイナミックに拍動する心筋細胞シートの表面電位の計測に成功している。さらに、ナノメッシュセンサのアクティブ化に成功し、物質透過性と高い生体適合性を兼ね備えつつ、伸縮自在で、高空間分解能・高時間分解能で計測できるナノメッシュセンサの開発を実現した。本年度は、薬効試験における定量的な評価に向けて、計測した表面電位の解析手法を確立し、表面電位、拍動数に加えて、細胞外電位持続時間の定量評価を推進した。また、心筋細胞の電気信号と伸縮力を同時に計測可能な柔らかいセンサを実現することで、ダイナミックに拍動する心臓モデルにおける興奮・収縮連関に関わる各指標を世界で初めて定量的に評価することに成功した。さらに、単一細胞より小さい素子サイズ(5×5 μm2)の有機電気化学トランジスタのマトリックス化を推進し、極めて低い電圧で細胞内電位を計測することに成功した。従来は、細胞内電位を計測する手法として、パッチクランプ法が用いられてきたが、侵襲性が大きいという課題とともに多点化が困難であるため、心筋細胞シートの活動電位の分布を計測することには適していなかった。一方で、電気穿孔法のように細胞に電気パルスを印加し、細胞内電位を計測する手法も提案されているが、電界集中で大きな印加電圧が必要であるため、細胞への侵襲性やプローブ電極の構造への制限が課題とされていた。そのため、細胞の生理電気的な信号の分布においては、多点電極の培養皿による表面電位分布といった細胞外信号のみに頼っていた。本研究により、心筋細胞の細胞内電位の分布を、1 V以下の低い電気信号のみで、高時間分解能で計測することが初めて可能になった。

Research Progress Status

令和3年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和3年度が最終年度であるため、記入しない。

  • Research Products

    (27 results)

All 2022 2021 2019 2018 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (20 results) (of which Int'l Joint Research: 20 results,  Invited: 20 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] 3D Printed Spring-Type Electronics with Liquid Metals for Highly Stretchable Conductors and Inductive Strain/Pressure Sensors2022

    • Author(s)
      Chihiro Okutani, Tomoyuki Yokota,Hiroki, Miyazako, and Takao Someya
    • Journal Title

      Advanced Materials Technologies

      Volume: - Pages: -

    • DOI

      10.1002/admt.202101657

    • Peer Reviewed
  • [Journal Article] Foundry-compatible high-resolution patterning of vertically phase-separated semiconducting films for ultraflexible organic electronics2021

    • Author(s)
      Binghao Wang, Wei Huang, Sunghoon Lee, Lizhen Huang, Zhi Wang, Yao Chen, Zhihua Chen, Liang-Wen Feng, Gang Wang, Tomoyuki Yokota, Takao Someya, Tobin J. Marks, and Antonio Facchetti
    • Journal Title

      Nature Communications

      Volume: 12 Pages: -

    • DOI

      10.1038/s41467-021-25059-8

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An organic transistor matrix for multipoint intracellular action potential recording2021

    • Author(s)
      Yasutoshi Jimbo, Daisuke Sasaki, Takashi Ohya, Sunghoon Lee, Wonryung Lee, Faezeh Arab Hassani, Tomoyuki Yokota, Katsuhisa Matsuura, Shinjiro Umezu, Tatsuya Shimizu, and Takao Someya
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 118 Pages: e2022300118

    • DOI

      10.1073/pnas.2022300118

    • Peer Reviewed / Open Access
  • [Journal Article] Simultaneous measurement of contractile force and field potential of dynamically beating human iPS cell-derived cardiac cell sheet-tissue with flexible electronics2021

    • Author(s)
      Takashi Ohya, Haruki Ohtomo, Tetsutaro Kikuchi, Daisuke Sasaki, Yohei Kawamura, Katsuhisa Matsuura, Tatsuya Shimizu, Kenjiro Fukuda, Takao Someya, and Shinjiro Umezu
    • Journal Title

      Lab on a Chip

      Volume: 21 Pages: 3899-3909

    • DOI

      10.1039/d1lc00411e

    • Peer Reviewed / Open Access
  • [Presentation] Electronic skins for the next- generation wearables2022

    • Author(s)
      Takao Someya
    • Organizer
      Tianjin Key Laboratory of Molecular Optoelectronics Sciences forum (TJ-MOS)
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins and the next- generation wearables for medical applications2022

    • Author(s)
      Takao Someya
    • Organizer
      IEEE EDS regions 9 and 10 Lecture
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins and the next- generation wearables for medical applications2022

    • Author(s)
      Takao Someya
    • Organizer
      innoLAE 2022
    • Int'l Joint Research / Invited
  • [Presentation] Electronic Skins for Robotics and Wearables2021

    • Author(s)
      Takao Someya
    • Organizer
      The 2021 International Symposium on VLSI Design, Automation and Test (2021 VLSI-DAT)
    • Int'l Joint Research / Invited
  • [Presentation] Nanomesh Based On Skin Electronics2021

    • Author(s)
      Tomoyuki Yokota, Sunghoon Lee, and Takao Someya
    • Organizer
      The Society for Information Display Virtual Display Week Symposium (2021 SID)
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins for medical and sports applications2021

    • Author(s)
      Takao Someya
    • Organizer
      Distinguished Medical Engineering Seminar
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins for robotics and wearables2021

    • Author(s)
      Takao Someya
    • Organizer
      Facebook Realith Labs
    • Int'l Joint Research / Invited
  • [Presentation] Skin electronics for medical applications2021

    • Author(s)
      Takao Someya
    • Organizer
      Future Materials 2021
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins for medical and sports applications2021

    • Author(s)
      Takao Someya
    • Organizer
      International Conference on "Materials for Humanity (MH 21)”
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins for biomedical applications2021

    • Author(s)
      Takao Someya
    • Organizer
      EML Webinar
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins for robotics and wearables2021

    • Author(s)
      Takao Someya
    • Organizer
      The 2nd International Conference on Flexible & Printed Optoelectronic Materials and Devices (ICFPOE 2021)
    • Int'l Joint Research / Invited
  • [Presentation] Skin electronics for medical applications2021

    • Author(s)
      Takao Someya
    • Organizer
      IEEE International Conference on Sensors and Nanotechnology (SENNANO 2021)
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins for robotics and wearables2021

    • Author(s)
      Takao Someya
    • Organizer
      Flex China 2021
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins for robotics and wearables2021

    • Author(s)
      Takao Someya
    • Organizer
      2021 Asian Solid-State Circuits Conference (A-SSCC)
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins and the next- generation wearables for medical applications2021

    • Author(s)
      Takao Someya
    • Organizer
      3rd International Conference on Flexible Electronics (ICFE)
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins for robotics and wearables2021

    • Author(s)
      Takao Someya
    • Organizer
      The 20th International Conference on Nanoimprint and Nanoprint Technologies 2021 (NNT2021)
    • Int'l Joint Research / Invited
  • [Presentation] Nanomesh pressure sensor without sensory interference2021

    • Author(s)
      Takao Someya, Sunghoon Lee, and Tomoyuki Yokota
    • Organizer
      Material Research Sosiety (2021 MRS Fall Meeting & Exhibit)
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins for robotics and its evolution to next generation wearables2021

    • Author(s)
      Takao Someya
    • Organizer
      CityU Croucher Advanced Study Institute
    • Int'l Joint Research / Invited
  • [Presentation] Electronic skins and the next- generation wearables for medical applications2021

    • Author(s)
      Takao Someya
    • Organizer
      KFPE conference which is organized by Korea Flexible Printed Electronics Society
    • Int'l Joint Research / Invited
  • [Presentation] Nanomesh Pressure Sensor Without Sensory Interference2021

    • Author(s)
      Takao Someya
    • Organizer
      Material Research Sosiety(2021 MRS Fall Meeting & Exhibit)
    • Int'l Joint Research / Invited
  • [Remarks] 染谷研究室ホームページ

    • URL

      http://www.ntech.t.u-tokyo.ac.jp/

  • [Patent(Industrial Property Rights)] ELECTRONIC FUNCTIONAL MEMBER AND ELECTRONIC COMPONENT2019

    • Inventor(s)
      Takao Someya and Sunghoon Lee
    • Industrial Property Rights Holder
      Japan Sci. and Tech. Agency
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      US11291114 B2
    • Overseas
  • [Patent(Industrial Property Rights)] 電子希望部材及び電子部品2018

    • Inventor(s)
      李成薫、染谷隆夫
    • Industrial Property Rights Holder
      国立研究開発法人科学技術振興機構
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      特許6960161

URL: 

Published: 2022-12-28  

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