2019 Fiscal Year Annual Research Report
Study of glioma infiltration in complex microenvironment on an automated microfluidic chip
| Project/Area Number |
17J00362
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
TSAI Hsieh Fu 沖縄科学技術大学院大学, 科学技術研究科, 特別研究員(DC1)
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| Project Period (FY) |
2017-04-26 – 2020-03-31
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| Keywords | hybrid microfluidics / glioblastoma / voltage-gated ionchannel / deep learning / neural network / cell migration / cell tracking |
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| Outline of Annual Research Achievements |
A. A two-layer hybrid microfluidic device is developed for high throughput electrotaxis study. A robust hybrid microfluidic device composed of PMMA/PDMS is developed with 2-layer design for high throughput electrotaxis study of glioblastoma. A method to uniformly seed sparse cells in microchannel is also achieved by balancing inlet/outlet pressure.
B. Electrotaxis of glioblastoma requires optimal laminin-containing extracellular matrix. Using the platform and the label-free cell tracking software we developed before, T98G and U-251MG glioblastoma electrotaxis are investigated and been found to depend on laminin extracellular matrix
C. Electrotaxis of glioblastoma cell models can be heterogenous. We have also found that T98G and U-251MG despite being molecularly and phenotypically typed similarly as mesenchymal glioblastoma, the electrotactic behavior is completely different. The result may reflect the heterogeneity of the brain cancer.
D. Electrotaxis of glioblastoma cells are mediated by voltage-gated ion channels. The calcium signaling plays a pivotal role in cell electrotaxis as well as various aspects of glioblastoma biology such as drug resistance and proliferation. We investigated if the voltage-gated calcium channels (VGCC) mediate the electrotaxis of glioblastoma and that different VGCCs are involved in different glioblastoma cell lines. Other ion channels such as voltage-gated potassium channels and the amiloride-sensitive sodium channels also mediates the glioblastoma cell electrotaxis. showing the importance of confirming patient-specific ion expression profile.
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| Research Progress Status |
令和元年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和元年度が最終年度であるため、記入しない。
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