detection of cyclic peptide from human blood after oral ingestion of protein hydrolysate

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K00906
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Tokyo Kasei Junior College
• Principal Investigator: Shigemura Yasutaka 東京家政大学短期大学部, 短期大学部, 准教授 (20373178)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: コラーゲン / 環状ペプチド / ヒドロキシプロリン

Outline of Final Research Achievements

The present study demonstrates the presence of a novel food-derived collagen peptide, cyclic Hydroxyproline containing (Hyp) peptides, in human blood and urine from 5 subjects after the ingestion of collagen hydrolysate. Among the peptides, cyclic Pro-Hyp was identified as major components and levels of that in plasma and urine samples were estimated by liquid chromatography mass spectrometry (LC-MS). Cyclic Pro-Hyp levels significantly increased in the plasma and urine after ingestion of collagen hydrolysate, reaching T max at 1.20 h and 2.00 h respectively. The maximum levels of cyclic Pro-Hyp in plasma and urine were 10.1 and 2.32 (mmol/mol Creatinine), representing approximately 5 and 2 % of linear Pro-Hyp in plasma and urine after ingestion of collagen hydrolysate respectively. Addition of cyclic Pro-Hyp in medium at 7 nmol/mL significantly enhanced the growth rate of mouse skin fibroblasts on collagen gel more extensively compared to linear Pro-Hyp.

Free Research Field

食品機能学

Academic Significance and Societal Importance of the Research Achievements

これまで食品中に環状ペプチドが存在することは知られていた。本研究では食品ペプチド摂取後に、それらが血中に吸収されて尿へと排出されることを初めて明らかにした。医薬品として環状ペプチドが流通しているが、食品としても機能性が明らかになれば食品への新たな付加価値の寄与に貢献が可能となる。また本研究から、摂取後に体内で食事由来環状ペプチドの濃度が増加し、被験者間で体内での食事由来環状ペプチド吸収もしくは形成が異なる可能性について見出した。これが環状ペプチドの機能性の個人差を及ぼすことも推測される。本成果から、今後の研究推進によって学術面だけでなく、健康寿命延伸に貢献できる社会的貢献にも大いに期待できる。