Elucidation of new metabolism promotion mechanism through extracellular vesicles derived from skeletal muscle

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K01843
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Mie University
• Principal Investigator: Kobayashi Yoshinao 三重大学, 保健管理センター, 教授 (70378298)
• Co-Investigator(Kenkyū-buntansha): 江口 暁子 三重大学, 医学系研究科, 特任講師(研究担当) (00598980)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 細胞外小胞 / メタボリック症候群 / 運動 / プロテオミクス解析

Outline of Final Research Achievements

We explored the association between circulating extracellular vesicles (EVs) level and various metabolic parameters, including obesity and lipid and glucose metabolisms, among 203 subjects with or without risk factor for metabolic diseases. Circulating EV number was significantly higher in men than in women. Circulating EV number correlated most strongly and significantly with elevated triglyceride. Circulating EVs represent a promising metabolic biomarker for lipid and glucose metabolism and have potential for monitoring metabolic status in humans, including individuals without metabolic risk factors.
In addition, we revealed that high intensity intermittent training exercise (HIIT-Ex) significantly influenced circulating EV number along with pulse rate, BP, and catabolism of skeletal muscle. Furthermore, HIIT-Ex promoted changes in EV protein composition, indicating that EVs intercommunicate across various organs in response to HIIT-Ex.

Free Research Field

健康増進

Academic Significance and Societal Importance of the Research Achievements

血液中の細胞外小胞の数および成分は、メタボリック症候群における全身の代謝異常を反映することが明らかになり、その時の健康状態を評価するための指標（バイオマーカー）になることが示された。
運動により血液中の細胞外小胞の数と構成タンパク質が変化することが明らかになり、構成タンパク成分の変化を通して運動により引き起こされる生体内の様々な代謝調節反応を評価することができ、健康増進に必要な運動の設定に役立つ情報が得られることが示された。