2020 Fiscal Year Final Research Report
Development of chemical tools for photocontrol of protein-protein interaction
| Project/Area Number |
17K05921
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Keywords | 光制御 / タンパク質二量体化 / 蛍光イメージング |
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| Outline of Final Research Achievements |
We aimed to establish photo-regulation techniques for protein dimerization in living cells. First, we developed a caged protein ligand for BL-tag by using a photolabile protecting group and succeeded in controlling the protein localization in a light irradiation-dependent manner in cells. We also developed a photochromic ligand for FKBP12(F36V) and found that the degree of protein dimer formation in the cell was changed by light irradiation.
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| Free Research Field |
ケミカルバイオロジー
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| Academic Significance and Societal Importance of the Research Achievements |
細胞内のタンパク質機能を人為的に操作する技術の開発が近年精力的に進められている。特に光を用いることで、時空間分解能の高い機能制御が可能になる。本研究で開発した光活性化型タンパク質二量化剤は、任意のタイミングで生細胞内のタンパク質間相互作用を光誘起可能である。本手法を用いて、今後、細胞内のシグナル伝達機構の詳細解析が促進され、疾患機構の解明につながると期待される。
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