Development of a pinpoint functionalization method of nucleic acids

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K05943
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Tokushima Bunri University
• Principal Investigator: Hari Yoshiyuki 徳島文理大学, 薬学部, 教授 (50347423)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: コンジュゲートオリゴ核酸 / オリゴ核酸上の化学反応

Outline of Final Research Achievements

In this research, iridium-conjugated oligonucleotides were synthesized and we found that the conjugated oligonucleotides could deiodinate (reduce) target oligonucleotides including iodouracil bases. Oligonucleotides bearing acylthio groups were also synthesized and it was achieved that acyl transfer from the acylthio group to the amino group of a target oligonucleotide where aminomethyluracil bases are contained. Moreover, the position suitable for acyl transfer was examined.

Free Research Field

核酸有機化学

Academic Significance and Societal Importance of the Research Achievements

狙った核酸の望みの位置の核酸塩基のみをピンポイントで直接的に官能基化することができれば、既存の機能性オリゴ核酸の更なる機能性向上や期待できます。本研究成果はそれを実現するためのはじめの一歩となる研究です。本研究で開発した可視光照射により酸化還元能を示すイリジウム連結オリゴ核酸やアシル基転位能を有する活性エステル連結オリゴ核酸は他の技術への応用も期待でき、今後の核酸化学研究の発展に寄与することが考えられます。