2019 Fiscal Year Final Research Report
Characterization and application of a novel dye-linked L-lactate dehydrogenase from thimophilic archaea
| Project/Area Number |
17K07748
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | L-乳酸 / 酸化還元酵素 / バイオセンサ / バイオ電池 |
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| Outline of Final Research Achievements |
Dye-linked L-lactate dehydrogenase (Dye-LDH) has high potential usefulness as a specific element for L-lactate biosensor and bio fuel cell. However, the practical application to Dye-LDH reported so far has been limited because of its low stability. We were screening for Dye-LDH with high stability from hyperthermophile. As a result, we found a novel Dye-LDH, and characterized the detail enzyme properties. In addition, investigation as a specific element for electrochemical devise was successful. These results indicate that the novel Dye-LDH derived from hyperthermophilic archaea has high potential utility for application to a specific element for electrochemical devices.
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| Free Research Field |
タンパク質化学
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| Academic Significance and Societal Importance of the Research Achievements |
本申請で見出した色素依存性L-乳酸脱水素酵素（Dye-LDH）は、L-乳酸をリアルタイムで測定可能なバイオセンサを開発するうえで非常に高い有用性を示すことが明らかとなった。L-乳酸は、様々な病気に対する重要なバイオマーカーになることが知られている。これまでに、L-乳酸をリアルタイムで測定可能なバイオセンサは、酵素の安定性の問題で開発が困難であった。本申請で見出したDye-LDHを用いてバイオセンサを構築できれば、これまで見出されているDye-LDHでは実現不可能であった長期間連続使用できるウェアラブルデバイスなど全く新しい用途で利用できるバイオデバイスの開発が可能となる。
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