2021 Fiscal Year Final Research Report
Development of micro total analysis system for biological compounds
| Project/Area Number |
17K08234
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Tsunoda Makoto 東京大学, 大学院薬学系研究科(薬学部), 准教授 (10323453)
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| Project Period (FY) |
2017-04-01 – 2022-03-31
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| Keywords | クロマトグラフィー / マイクロ流体デバイス |
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| Outline of Final Research Achievements |
A technology to enable quantification of trace amounts of biological compounds using pillar array columns as a high-performance on-chip liquid chromatography separation medium was developed. So far, fundamental technologies for separation of biological compounds on pillar array columns have been established. In this study, the sample injection part was investigated as one of the fundamental technologies for quantitative analysis. An automated sample injection system was developed with good reproducibility. Reproducible quantification of amino acids in plasma samples has become possible.
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| Free Research Field |
生体分析化学
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| Academic Significance and Societal Importance of the Research Achievements |
オンチップ液体クロマトグラフィー技術を用いた生体試料中低分子化合物定量への応用は、これまで、限定されていた。本研究成果により、オンチップ液体クロマトグラフィーの中でも高性能な分離が可能であるピラーアレイカラムを用いた生体分子定量が可能となった。ピラーアレイカラムは高分離能を有することから、生体分子の迅速分析による疾病診断等への応用が期待される。
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