Application of the strategy for nucleic acid delivery aimed at remote control of intracellular dynamics to the therapy

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K08238
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Setsunan University
• Principal Investigator: Ukawa Masami 摂南大学, 薬学部, 助教 (50735511)
• Co-Investigator(Kenkyū-buntansha): 佐久間 信至 摂南大学, 薬学部, 教授 (80388644)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 核酸デリバリー / 膜透過ペプチド / プラスミドDNA

Outline of Final Research Achievements

Nucleic acids are applicable for the therapy of various disease, but carriers are required to cellular delivery of nucleic acids. We considered that the polymer, which is designed based on the notion of the enhancement of cellular uptake of drugs on the surface on the cells, could be suitable for the carrier. Furthermore, we hypothesized that the dynamics of nucleic acid after the uptake could be controlled by other drugs. As a result, the enhancement of transgene expression by addition of other drugs was observed in the tumor cell lines. It was also implied that cellular uptake and translocation of genes into nucleus were effected by other drugs.

Free Research Field

製剤学

Academic Significance and Societal Importance of the Research Achievements

一般に徐放性・根本的治療を目的とする核酸医薬やDDS 製剤と、即効性・対症療法を得意とする低分子薬物との組み合わせは、実際の疾患の治療においても併用薬として相性が良いことが考えられる。本研究において得られた核酸と併用薬の相互作用の情報は、オリゴアルギニン固定化高分子を用いた導入法のみならず、他のキャリアを用いた核酸医薬においても重要な薬物相互作用情報となりうると考えている。