The molecular mechanism for the generation of ROS through Nox

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K08637
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kawasaki Medical School (2018-2019); Kyushu University (2017)
• Principal Investigator: Miyano Kei 川崎医科大学, 医学部, 助教 (60444783)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: Nox / 活性酸素 / 細胞遊走 / 上皮細胞 / 殺菌

Outline of Final Research Achievements

Superoxide producing NADPH oxidase 1 (Nox1) that expresses abundantly in colon epithelium plays a crucial role in mucosal host defense. Previous studies have shown that Nox1 participates in epithelial migration which is an important process in mucosal wound healing. In this study, we investigated the effect of ROS scavenger’s pre-treatment on migration of human colon cancer HCT116 cells, which express Nox1. We found that Nox1 activity is positively feedbacked by its product itself, then the increased products depend on the regulation of migration ability of the cells.

Free Research Field

生化学

Academic Significance and Societal Importance of the Research Achievements

活性酸素は基本的に生体に有害であるため、Nox1の制御機構の破綻は、消化管上皮の疾患を引き起こす可能性がある。実際に、大腸がんの増悪とNox1の過剰な活性化の関連が指摘されており、どのようなメカニズムでNox1活性が調節を受けるのか、また、活性酸素がどのような分子メカニズムで遊走を制御しているのかを明らかにすることは重要な課題であった。今回の研究成果は、酸化ストレスにより引き起こされる様々な疾患の原因を明らかにする上で役立つものと考えられる。