Physsiological role of LPL in sdLDL production

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K08974
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Gunma University
• Principal Investigator: Kimura Takao 群馬大学, 大学院医学系研究科, 准教授 (90396656)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 脂質代謝 / 中性脂肪 / レムナント / sdLDL / LPL / GPIHBP1 / HTGL

Outline of Final Research Achievements

The LPL playing a central role of the lipid metabolism exists on the blood vessel wall by GPIHBP which is anchor protein and promotes metabolism of RLP-C and sdLDL-C. The LPL concentration was positively correlated with HDL-C, and inversely correlated with TG and RLP-C. The HTGL concentration was positively correlated with RLP-C and sdLDL-C. The HTGL ratio of the pre-heparin/post-heparin plasma concentration and sdLDL-C/LDL-C ratio were significantly greater in coronary artery disease (CAD) patients than in non-CAD patients. GPIHBP1 was positively correlated with LPL and inversely correlated with RLP-C and sdLDL-C. The HTGL concentration was positively correlated with RLP-C and sdLDL-C, while LPL and GPIHBP1 were inversely correlated with RLP-C and sdLDL-C. These results suggest that elevated HTGL is associated with increased CAD risk, while elevated LPL is associated with a reduction of CAD risk.

Free Research Field

脂質代謝

Academic Significance and Societal Importance of the Research Achievements

我々はこれまでヘパリン投与が必要であった血中のLPLとHTGL濃度の評価がヘパリン投与なしで可能になる測定系を開発し、短時間で大量の検体のLPLおよびHTGL濃度の測定を可能にした。この測定系を利用して冠動脈疾患の患者では冠動脈疾患の無い患者に比べHTGLが高いこと、更にLPLとそのアンカー蛋白であるGPIHBP1が高いほど、冠動脈疾患の原因となる脂質であるsdLDLやRLP-Cの血中濃度が低いことを明らかにした。冠動脈疾患のリスクを減少させるには血中のLPL濃度を高くし、HTGL濃度を低くすることが重要であることが示唆された。