Mechanism of production, secretion and hypertension in the endogenous digitalis like factors mediating lipolytic enzyme

2021 Fiscal Year Final Research Report

• Project/Area Number: 17K08997
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kansai Medical University
• Principal Investigator: YOSHIKA Masamichi 関西医科大学, 医学部, 非常勤講師 (70434834)
• Co-Investigator(Kenkyū-buntansha): 保坂 直樹 関西医科大学, 医学部, 研究員 (30388459)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Keywords: 内因性ジギタリス様物質 / マリノブファゲニン / マリノブフォトキシン / エステラーゼ / ELISA / HPLC

Outline of Final Research Achievements

This research is a part of patho-physiological study of endogenous digitalis-like factors (EDLFs). We took notice of the maronobufotoxin (MBT) in special structure having both peptide and ester bond. We confirmed MBT was converted to marinobufagin incubated with trypsin or any enzymes　concerned with congealing fibrinogenolysis system. Those enzymes have cleavage activities of peptide or ester bond.
Further studies on the types of enzymes and reaction conditions are required, but our studies will lead to the elucidation of the production mechanism of EDLFs, which has not yet been fully elucidated.

Free Research Field

医歯薬

Academic Significance and Societal Importance of the Research Achievements

高血圧の発症はさまざまな要因が存在するが、その１つとして内因性ジギタリス様物質（EDLF）がナトリウム代謝を介して高血圧発症に関わると考えられている。EDLFには多くの候補物質が存在するがどの候補物質も産生分泌機序が十分に解明されていない。
今回の研究結果内因性ジギタリス様物質の産生メカニズムの解明につながり、ひいては新規機序の高血圧治療薬の開発につながる基礎的検討として意義があると考える。