Mixture analysis and age estimation based on mitochondrial DNA polymophisms and methylation pattern

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K09259
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Asahikawa Medical College
• Principal Investigator: Masaru Asari 旭川医科大学, 医学部, 准教授 (40360979)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 個人識別 / 混合試料 / ミトコンドリアDNA / メチル化

Outline of Final Research Achievements

We developed a new method for discrimination of mitochondrial DNA haplotypes based on inhibition of amplification using designed probes including single nucleotide polymorphisms or reference sequences. Our method could effectively discriminate each haplotype from mitochondrial DNA mixtures. Although we did not find the useful methylation targets for age estimation of Japanese individuals, we confirmed that single bese extention method would be useful to detect lower methylation rates.

Free Research Field

法医学

Academic Significance and Societal Importance of the Research Achievements

本研究結果は、きわめて微量な混合試料からのミトコンドリアDNA型解析法として法医実務へ利用可能である。特に検体数が多い場合においても、効率よく被疑者および被害者など含まれる個人のミトコンドリアDNA型が検出可能であると考える。