Elucidation of mechanisms of cancer progression by exosome derived from hematopoietic cells

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K10601
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: University of Yamanashi
• Principal Investigator: ICHIKAWA Daisuke 山梨大学, 大学院総合研究部, 教授 (20347446)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 消化器癌 / exosome

Outline of Final Research Achievements

Uptake of red blood cell- (RBC) and platelet-derived exosomes into gastric cancer and mesothelial cell lines were confirmed. Increased migration ability was confirmed in cell lines with addition of the RBC-derived exosomes compared with control. Similarly, addition of platelet-derived exosomes resulted in increase of migration ability of gastric cancer cell lines. Further PCR-array based analysis demonstrated that addition of the platelet-derived exosomes generated alteration in some gene expressions in gastric cancer cell lines. On the other hand, co-culture of gastric cancer cell lines with platelet generated adherence of platelet to cancer cells, and the co-culture led increases of proliferation, migration and invasion ability of gastric cancer cell lines.

Free Research Field

消化器外科

Academic Significance and Societal Importance of the Research Achievements

本研究によって、無核である血球細胞に含まれる様々な遺伝情報が細胞外に分泌され、それらが受け側細胞に取り込まれることによって、様々な機能変化を来たすことが判明した。実臨床では、担癌状態の末期には様々な血球の異常が認識されており、外科手術においては、術野への多量の血液が暴露することも周知の事実である。これら血球細胞の癌進展への関与についての更なる分子機序の解明によって、全く新たな治療法の開発が期待され、依然、治療成績が不良である高度進行癌患者に対して極めて大きな福音となると考えられる。