Investigation of polybacterial onset model of tonsillitis repetition and severity by crowd analysis of tonsil crypt microflora

2023 Fiscal Year Final Research Report

• Project/Area Number: 17K11400
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Wakayama Medical University
• Principal Investigator: Masanobu HIRAOKA 和歌山県立医科大学, 医学部, 助教 (80423945)
• Co-Investigator(Kenkyū-buntansha): 杉田 玄 和歌山県立医科大学, 医学部, 博士研究員 (20407274) ; 河野 正充 和歌山県立医科大学, 医学部, 准教授 (20511570) ; 保富 宗城 和歌山県立医科大学, 医学部, 教授 (90336892)
• Project Period (FY): 2021-01-01 – 2024-03-31
• Keywords: microbiome / 16S / tonsil / 菌叢解析 / 扁桃炎

Outline of Final Research Achievements

In order to analyze the bacterial flora in the crypts of patients with recurrent and severe tonsillitis and to provide new insights into a multibacterial model of tonsillitis pathogenesis, debris samples were collected bilaterally from 41 tonsillectomy cases by swabbing the surface of the tonsils and aspirating from the deep crypts. Tissue fragments were also collected from surgical specimens and from the deep crypts inhabiting the tonsil surface and the deep crypts, and DNA extraction and sequence analysis using a next-generation sequencer with UCSD Microbiome Core were performed. Sequence information was uploaded to Qiita, a cloud service.

Free Research Field

菌叢解析

Academic Significance and Societal Importance of the Research Achievements

扁桃炎反復の機序解析を目的として、我々は、歯周病原菌とされる嫌気性菌Red Complexがsupporterとして陰窩に慢性炎症状態を作りだし、宿主粘膜上皮に細胞内寄生した化膿レンサ球菌(GAS)などのpathogenの増殖、組織内侵入を促し、二者を抑制するcompetitor乳酸菌との相互作用により発病に至るという多細菌性発症モデルを想定し実験をおこなった。この成果は陰窩洗浄などの抗biofilm治療やprobioticsの扁桃炎予防効果の根拠となると考える。