Involvement of 12 / 15-LOX with intracellular organelle degradation mechanism in root resorption

2020 Fiscal Year Final Research Report

• Project/Area Number: 17K11943
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Nagasaki University
• Principal Investigator: HOTOKEZAKA Hitoshi 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (90199513)
• Co-Investigator(Kenkyū-buntansha): 森田 幸子 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (00631574) ; 坂井 詠子 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10176612) ; 根本 孝幸 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (90164665)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Keywords: ortohdontic / root resorption / 12/15-LOX / lipoxygenase

Outline of Final Research Achievements

A closed coil spring was attached between the maxillary first molar and the incisors of the rat, and the maxillary first molar was moved mesially. A 12 / 15-LOX inhibitor (baicalein) was intraperitoneally administered daily. On day 14, the tooth was extracted, and the tooth root absorption area was measured with a scanning electron microscope (SEM). The root absorption depth was measured with a scanning laser microscope (SLM), and the root absorption volume was calculated. As a result, it was 7.7±4.8ｘ10(7) μm(3) in the control, while it was 2.2±0.8ｘ10(7)μm(3) in the 12 / 15-LOX inhibitor administration group. There was a significant difference between the two groups (p <0.01).

Free Research Field

歯科矯正学

Academic Significance and Societal Importance of the Research Achievements

矯正歯科治療の需要は年々高まっているが、矯正歯科治療の重大な副作用として歯根吸収が問題で早期の解決が求められている課題である。近年、歯根吸収発症の過程に、歯根膜組織の硝子様変性や炎症が深く関与している可能性が明らかになってきたが、歯根吸収の予防薬は未だ開発されていない。我々は、アラキドン酸代謝酵素である 12/15-リポキーシゲナーゼ(12/15-LOX)が歯根膜の炎症反応を誘導する働きがある事に注目し、12/15-LOX 歯根吸収の発症に関わると考え、その阻害剤を用いて予防薬の開発を試みた。