Comprehensive assessment of serum vitamin D metabolism by LC/MS/MS

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K15768
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Chiba University
• Principal Investigator: Ishige Takayuki 千葉大学, 医学部附属病院, 臨床検査技師 (30757315)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: ビタミンD代謝物 / 質量分析 / 遺伝子多型 / 融解曲線分析

Outline of Final Research Achievements

Vitamin D has various roles related to bone metabolism and immune regulation. To elucidation of vitamin D metabolism, the accurate quantification method of its metabolites is required. In the circulating blood, vitamin D and its metabolites bind to vitamin D-binding protein (DBP), encoded by GC gene. In this study, we developed accurate quantification methods of vitamin D metabolites using LC/MS/MS. We also developed a high-throughput GC genotyping method using melting analysis with locked nucleic acid-incorporating dual hybridization probe for improving mismatch discrimination.

Free Research Field

医歯薬学

Academic Significance and Societal Importance of the Research Achievements

健常者におけるビタミンD代謝物の血清濃度とGC遺伝子多型の詳細な関連が明らかになり、各種疾患とビタミンDの関連を理解するのに有用になることと思われる。LC/MS/MSを用いた高感度かつ高精度な分析法の構築により、ビタミンD代謝動態がより精確に把握できるようになる。臨床検査としても利用することが可能と思われ、LC/MS/MSを用いることで測定費用を低く抑えることができ、放射性廃棄物の問題も軽減できる。