Functional analysis of enhancers to dissect differential roles of BMP7 secreted from distinct sites in kidney.

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K16072
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Kyoto University (2019); Keio University (2017-2018)
• Principal Investigator: Tsujimura Taro 京都大学, 高等研究院, 特定講師 (90741893)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: エンハンサー / BMP7 / 腎臓 / CTCF

Outline of Final Research Achievements

Regarding the enhancer regulation for the Bmp7 expression in kidneys, this study shows that i) the downstream region of Bmp7 does not contain enhancers that are critical for the normal kidney development; ii) the Bmp7 expression in the adult kidney is limited to only small population of cells; iii) the CTCF binding sites at the locus for sure critically impinge on the enhancer regulation; iv) however, the role for the CTCF binding sites specifically in the kidney cannot be ensured unless the precise position of the enhancer is identified and the epigenetic states around the region are revealed.

Free Research Field

エンハンサー制御

Academic Significance and Societal Importance of the Research Achievements

Bmp7の制御という観点からは、より理解を深めていくために、シングルセルレベルでの詳細な解析が必要になってくることを示唆した。エンハンサー制御に関連して得たCTCFの機能およびその調節動態についての知見は、他の遺伝子座においても同様であるはずで、腎臓のみならず広く生体機能をゲノムから探る上での大きな指針となる研究になったと考えている。