Pathophygiological Investigations of ATRAP in Renal Tubules on Kidney Injury

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K16091
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Yokohama City University
• Principal Investigator: Uneda Kazushi 横浜市立大学, 医学部, 助教 (90780370)
• Research Collaborator: Tamura Kouichi ; Wakui Hiromichi ; Kinguchi Sho
• Project Period (FY): 2017-04-01 – 2019-03-31
• Keywords: レニン・アンジオテンシン系 / 腎内レニン・アンジオテンシン系 / ATRAP / 高血圧 / 慢性腎臓病

Outline of Final Research Achievements

In this study, we generated proximal tubule-specific ATRAP KO mice. Detailed analysis of renal ATRAP expression revealed that ATRAP mRNA expression was decreased by nearly 80% in proximal regions of the nephron in proximal tubule-specific ATRAP KO mice compared with wild-type mice. There were no significant differences in blood pressure, urinary sodium retention and kidney damages between proximal tubule-specific ATRAP KO mice and wild-type mice under conditions of chronic angiotensin II stimulation. These results indicate that renal proximal tubule ATRAP has a minor role in angiotensin-mediated hypertension or kidney injury.

Free Research Field

腎臓

Academic Significance and Societal Importance of the Research Achievements

今回の検討により，近位尿細管特異的ATRAP欠損マウスではアンジオテンシンII依存性高血圧・腎障害の増悪を認めないことが明らかとなった．尿細管のATRAPに関する既報と今回の結果を総合すると，近位尿細管におけるATRAPがアンジオテンシンII依存性高血圧・腎障害の病態に及ぼす影響は少なく，むしろ遠位尿細管に存在するATRAPが重要である可能性が想起された．