ENGRAFTMENT POTENTIAL OF MATERNAL ADIPOSE-DERIVED STEM CELLS FOR IN UTERO TRANSPLANTATION

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K16311
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Showa University
• Principal Investigator: Kawashima Akihiro 昭和大学, 医学部, 講師 (10783376)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 脂肪由来間葉系幹細胞 / 胎児治療 / 幹細胞治療

Outline of Final Research Achievements

We hypothesised that maternal mesenchymal stem cells (MSCs) might be a better cell source of in utero transplantation for the fetal tissue than allograft MSCs. In the first part, the allograft ADSCs after in utero transplantation settled at day 28 after transplantation and also expressed TUBB3 and NeuN. In the second part of this study, we demonstrated that the maternal ADSCs induced less CD45+ cells and GFAP+ astrocytes around the transplanted cells than allogenic ADSCs. The maternal ADSCs expressed less TNFα, IL-6 and TIM1 gene expression in the fetus brain than allogenic ADSCs. Transplanting ADSCs from the mother may improve the success of in utero allogenic transplantation of ADSCs for their immune tolerance to the fetus.

Free Research Field

周産期医学

Academic Significance and Societal Importance of the Research Achievements

この成果はダウン症児の高次機能障害の改善を目的とする胎児治療において、母体の間葉系幹細胞を用いることでより移植後の免疫反応を特別な加療なく抑制させることから道を開く画期的な方法であると考える。本研究成果は、出生前診断により早期治療介入が可能となった染色体疾患や遺伝性疾患をもつ胎児に対して、胎児期より進行する中枢神経系疾患における胎児治療の可能性を示すものである。