Analysis of metabolic remodeling in amino acids in glioblastoma cells

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K16648
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Kobe University
• Principal Investigator: Nakamizo Satoshi 神戸大学, 医学研究科, 医学研究員 (00569238)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Keywords: 膠芽腫 / グルタミン / セリン / 代謝リモデリング

Outline of Final Research Achievements

Core of the glioblastoma (GBM) tissue had nutrient starvation. As compared with edge of the GBM, core of the GBM had low concentration of glutamine and high concentration of serine, which was brought by a metabolic remodeling of amino acid. Serine metabolic enzyme, PSAT1, SHMT2, and MTHFD2 were increased in GBM tissues, especially, in the area nearby necrotic regions. Cell proliferation and cell cycle was inhibited by knockdown of PSAT1. In addition, knockdown of PSAT1 induced apoptosis in glioma cells. Our study suggested that the amino acid metabolic remodeling pathway and remodeling enzymes may be useful therapeutic targets in GBM therapy.

Free Research Field

脳腫瘍

Academic Significance and Societal Importance of the Research Achievements

近年、質量分析器などの機器の向上で、がん細胞での代謝リモデリングが細かなことまで明らかとなってきている。しかし、セリンに注目した研究はまだ端緒についたばかりである。膠芽腫におけるセリン代謝が明らかとなれば、新たな治療法の開発につながる可能性もあり、臨床的にも意義ある研究と思われる。