Development of new bone mass maintenance type bone formation technology using epigenetic change

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K17169
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Okayama University
• Principal Investigator: Yoshioka Yuya 岡山大学, 大学病院, 医員 (20782014)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Keywords: DNMT3A / 遺伝子A, 遺伝子B

Outline of Final Research Achievements

We analyzed methylation patterns using a next-generation sequencer, and identified transcription factor X, which is promoted by methylation when induced to induce osteoblast differentiation, and transcription factor Y, which is suppressed when DNMT3A is overexpressed.
However, we examined the effects of transcription factors X and Y on osteoblast differentiation in vitro, but we did not obtain expected results.
Therefore, we focused on the genes A and B, which are factors involved in undifferentiated maintenance, by changing the focus point, and examined the influence of DNMT3A on the genes A and B in vitro. As a result, it was clarified that DNMT3A promotes the methylation of the promoter region of genetic day A and gene B in vitro and suppresses the gene expression.

Free Research Field

骨・軟骨代謝

Academic Significance and Societal Importance of the Research Achievements

本研究成果によりDNMT3Aは未分化維持関連因子の遺伝子A, 遺伝子Bのプロモーター領域の遺伝子Bのプロモーター領域のメチル化を促進し，その遺伝子発現を抑制することが明らかとなった。このことによりin vitroにおいてDNMT3Aは上記のメカニズムを通じて骨芽細胞分化をさらに促進する可能性が示唆された。本研究成果は今後，骨量を維持できる全く新しい骨造成技術の開発に精通する可能性が示唆される。