Regulation of cyclin D1 gene expression by lncRNA transcribed from its promoter region

2020 Fiscal Year Final Research Report

• Project/Area Number: 17K18065
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology; Cell biology
• Research Institution: Saitama Medical University
• Principal Investigator: YONEDA Ryoma 埼玉医科大学, 医学部, 助教 (00734881)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Keywords: RNAメチル化 / lncRNA / pncRNA-D / TLS/FUS

Outline of Final Research Achievements

m6A modification of lncRNA named pncRNA-D regulates cell cycle by controlling the expression of cyclin D1.
RNA is also methylated or acetylated, and this time I focused on the most abundant RNA modification, m6A. pncRNA-D is a long noncoding RNA transcribed from the promoter region of cyclin D1. m6A modification of pncRNA-D altered interaction with RNA binding proteins, and half-life of this RNA. in summary, I discovered m6A modification of pncRNA-D is deeply related with cell cycle and cell growth of cancer cell lines.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では複数のがん細胞由来の細胞株の増殖を抑えるRNA修飾を発見した。これはつまり、がん細胞間で保存された細胞増殖メカニズムを抑制するRNA修飾であることを意味する。本研究をさらに進めると、多くのがん細胞の増殖を抑えられるRNA断片の作製に繋がることが期待される。