Development and regenerative research of a novel primate model of cerebral infarction

2020 Fiscal Year Final Research Report

• Project/Area Number: 17K18130
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science; Experimental pathology
• Research Institution: Jikei University School of Medicine
• Principal Investigator: Komatsu Teppei 東京慈恵会医科大学, 医学部, 助教 (60723856)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Keywords: 脳梗塞 / 動物モデル / 再生医療 / 細胞治療 / 脳血管造影 / マーモセット / ラット / MRI

Outline of Final Research Achievements

We have succeeded in creating a rat cerebral infarction model by percutaneous tail artery puncture using a digital subtraction angiography system to realize regenerative medicine for post-stroke syndrome. It can reproduce middle cerebral artery territory cerebral infarction in a minimally invasive and short time. Since we can repeatedly perform cerebral angiography by placing a catheter in the caudal artery, it is possible to evaluate occluded vessels, recanalization, vascular regeneration, and transarterial therapeutic intervention. By applying this technology, we have successfully performed cerebral angiography of common marmosets. We have also succeeded in creating radiopaque cell-encapusulated hydrogel microfibers that can be applied to regenerative therapy. It enables long-term retention of cells after intravitreal administration, and is expected to have a long-term paracrine effect by cytokines. We have applied for a patent for this technology.

Free Research Field

脳梗塞

Academic Significance and Societal Importance of the Research Achievements

脳は一度損傷を受けると再生しないため脳卒中は治らない病気であり、寝たきり原因の第一位である。脳梗塞後遺症に対する再生医療が期待され、経動脈的細胞投与を検証可能な動物モデルの開発が急務であり、本研究において確立した。また経動脈的に投与可能かつX線を撮像するのみでモニタリング可能、そして長期生存できるファイバー形状の細胞を開発した。本技術は既存治療では克服出来ない脳梗塞や慢性腎障害、大動脈血管障害、心筋梗塞、肝不全などの多くの疾患の再生治療を行うことが可能であり、超高齢社会のニーズに応えることが出来る。