Construction of Integrated Nucleic Acid Drug Production and Delivery System Based on Glycovesicles

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K19030
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Nano/Micro science and related fields
• Research Institution: Kyoto University
• Principal Investigator: Nishimura Tomoki 京都大学, 工学研究科, 特定助教 (60648070)
• Project Period (FY): 2017-06-30 – 2019-03-31
• Keywords: ポリマーベシクル

Outline of Final Research Achievements

In this study, we synthesized nucleic acid medicine, which is attracting attention as a next-generation drug, by a rolling circle amplification in molecular permeable polymer vesicles that we developed previously. In the course of conducting this study, we also found that amphiphilic block polymer composed of carbohydrate and poly (n-propyl oxazoline), which shows a lower critical solution temperature around room temperature, self-assembled into molecular permeable polymer vesicles. The introduction of hydrophobic polymers showing LCST-like behavior such as poly(n-propyl oxazoline)s as a hydrophobic segment represents a new approach to constructing permeable polymer vesicles. Our study thus opens up an avenue for designing and controlling the molecular permeability of polymer vesicles.

Free Research Field

自己組織化材料化学

Academic Significance and Societal Importance of the Research Achievements

本研究は、我々が独自に開発した分子透過を示す高分子ベシクルの性質を生かし、高分子ベシクル内での酵素反応により核酸医薬産生を行なった。これにより、従来の核酸送達キャリアが持つ封入率、毒性及び安定性に関する問題を克服する足がかりになると考えられる。また、水和能を有するpoly(n-propyl oxazoline)が、ポリマー二分子膜の分子透過性を向上させることが明らかとなり、分子透過能性高分子ベシクルの設計指針に結びつくと考えられる。