2021 Fiscal Year Final Research Report
Development of a peptide selection system to modulate polymerase functions
| Project/Area Number |
17K19212
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry and related fields
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Tagami Shunsuke 国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (40586939)
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| Project Period (FY) |
2017-06-30 – 2020-03-31
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| Keywords | ペプチド / ポリメラーゼ |
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| Outline of Final Research Achievements |
In this study, we attempted to develop a peptide selection system to convert polymerases into nucleases. For this purpose, we modified the mRNA display method and realized screening in which peptides were recovered only when a nuclease reaction occurred. We aim to obtain useful peptides by applying this screening system to various polymerases in the future.
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| Free Research Field |
合成生物学
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| Academic Significance and Societal Importance of the Research Achievements |
様々な病原体（ウィルス，がん細胞など）の増殖にはその遺伝情報を増幅するためのポリメラーゼが必要です．本研究ではそのようなポリメラーゼの機能を変換し，むしろ病原体の遺伝情報を破壊するようにするためのペプチド開発を進めています．研究期間中に複雑なペプチドスクリーニング法の改変を行いましたが，今後これを利用して様々な有用ペプチドの取得を目指しています．
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