• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2018 Fiscal Year Final Research Report

DNA injection by agrobacteria strengthened with gram-positive bacteria

Research Project

  • PDF
Project/Area Number 17K19240
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Agricultural chemistry and related fields
Research InstitutionHiroshima University

Principal Investigator

Suzuki Katsunori  広島大学, 理学研究科, 教授 (50221320)

Project Period (FY) 2017-06-30 – 2019-03-31
Keywordsグラム陽性菌 / アグロバクテリア / 広域DNA伝達 / T-DNA輸送 / プラスミド
Outline of Final Research Achievements

Mobilizable plasmids were designed with an aim to transfer long size DNAs to a wide range of organisms taking the advantage of Agrobacterium’s special ability to transform various organisms. A wide host range mobilizable plasmid functional in G(+) bacteria was combined with the origin of T-DNA transfer RB together with a wide host range replication gene for G(-) bacteria and yeast genes. The resultant plasmid was found able to shuttle bacteria and transferable from Agrobacterium to yeast strains. This study also indicated transportation of a model T-DNA plasmid to Escherichia coli strains.

Free Research Field

微生物遺伝学

Academic Significance and Societal Importance of the Research Achievements

アグロバクテリアのT-DNA輸送機構によって大腸菌へモデルT-DNAプラスミドを伝達できることを示した成果はアグロバクテリのT-DNA輸送伝達域を拡張したに留まらず、T-DNA輸送機構の細菌接合装置起源とその後の分化研究の端緒となると共に、大腸菌の豊富な資源を利用した受容細胞側の分子機構解明を可能にした。
モデルT-DNAプラスミドにグラム陽性菌の広域複製および伝達機能を付加したシャトルプラスミドを初めて作成し酵母菌へ輸送した成果は、長い外来DNAをアグロバクテリアで扱い種々の生物へ輸送するためのベクターツールの先駆けとなる。

URL: 

Published: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi