TCR sequencing of tumor infiltrating T cells using iPS cell technology

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K19696
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Surgery of the organs maintaining homeostasis and related fields
• Research Institution: Nagoya University
• Principal Investigator: Naoe Yoshinori 名古屋大学, 医学系研究科, 特任准教授 (50392048)
• Co-Investigator(Kenkyū-buntansha): 粕谷 英樹 名古屋大学, 医学系研究科, 教授 (00402636)
• Project Period (FY): 2017-06-30 – 2019-03-31
• Keywords: 細胞障害性T細胞 / iPS細胞 / 癌免疫

Outline of Final Research Achievements

By collecting tumor-specific cytotoxic T cells infiltrating the mouse tumor and introducing Yamanaka factor, it was possible to obtain a cell population that proliferates without anti-CD3 antibody stimulation. These cells were judged as iPS cells because they proliferated without TCR stimulation. In addition, we confirmed a gene rearrangement of the TCRb in all the proliferating cells examined. From the above, it was suggested that cells proliferating without anti-CD3 stimulation are derived from T cells. In addition, we tried to establish iPS cells using human peripheral cytotoxic T cells, but the establishment efficiency was low. In the future, we will investigate culture conditions and try to improve the establishment efficiency.

Free Research Field

免疫学

Academic Significance and Societal Importance of the Research Achievements

本研究により、腫瘍に浸潤したT細胞にin vitroで山中因子を導入しiPS細胞の樹立に成功した。このことから、iPS細胞化したT細胞は無限増殖能を獲得することにより、個々のT細胞のクローニングが容易となり、腫瘍に浸潤した個々のT細胞のTCRを同定することが可能となった。この技術を利用することにより、新規がん抗原ならびにがん抗原を認識するTCRの探索研究が進むと考えられる。