2010 Fiscal Year Final Research Report
Basic study to establish "humanized animal model" by improvement and modification of severely immunodeficient NOG mouse
| Project/Area Number |
18100005
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Central Institute for Experimental Animals |
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Principal Investigator |
ITO Mamoru Central Institute for Experimental Animals, 免疫研究室, 室長 (00176364)
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| Co-Investigator(Kenkyū-buntansha) |
HABU Sonoko 東海大学, 医学部, 教授 (30051618)
ANDO Kiyoshi 東海大学, 医学部, 教授 (70176014)
MIYAKAWA Yoshitaka 慶応義塾大学, 医学部, 講師 (50250238)
SUZUE Kazutomo 群馬大学, 医学研究科, 講師 (00333485)
SUEMIZU Hiroshi (財)実験動物中央研究所, 分子解析研究室, 室長 (40332209)
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| Project Period (FY) |
2006 – 2009
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| Keywords | 病態モデル / 免疫不全マウス / ヒト化動物モデル |
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| Research Abstract |
Severe immunodeficient NOG mice reported by us in 2002 show extremely high engraftment potential for xenogenic cells and tissues. Therefore, these mice enable us to generate "humanized mice" in which human cells and tissues act in the same way in humans by transplantation of human cells and tissues into NOG mice. The objective of this project is to improve immunodeficient mice including NOG mice by introducing human genes or by inactivating mouse genes to facilitate the generation of humanized mice. In the 4-year project period, we have newly established 33 strains of immunodeficient mice, and are working on 10 strains are in progress. By using NOG mice and these improved immunodeficient mice, we established various humanized models including those for infectious diseases, GVHD, cancer metastasis, human liver metabolism and stem cell differentiation. These models may be useful to evaluate new drug efficacy and safety, and also to analyze the mechanisms underlying human diseases.
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[Journal Article] Selective infection of CD4+ effector memory T lymphocytes leads to preferential depletion of memory T lymphocytes in R5 HIV-1-infected humanized NOD/SCID/IL-2Rgammanull mice.2009
Author(s)
Nie C., K.Sato, N.Misawa, H.Kitayama, H.Fujino, H.Hiramatsu, T.Heike, T.Nakahata, Y.Tanaka, M.Ito, Y.Koyanagi
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Journal Title
Virology 394
Pages: 64-72
Peer Reviewed
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[Journal Article] A new humanized mouse model of Epstein-Barr virus infection that reproduces persistent infection, lymphoproliferative disorder, and cell-mediated and humoral immune responses.2008
Author(s)
Yajima M., K.Imadome, A.Nakagawa, S.Watanabe, K.Terashima, H.Nakamura, M.Ito, N.Shimizu, M.Honda, N.Yamamoto, S.Fujiwara
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Journal Title
J Infect Dis 198
Pages: 673-682
Peer Reviewed
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[Journal Article] Development of human Graafian follicles following transplantation of human ovarian tissue into NOD/SCID/gammacnull mice.2008
Author(s)
Terada Y., Y.Terunuma-Sato, T.Kakoi-Yoshimoto, H.Hasegawa, T.Ugajin, Y.Koyanagi, M.Ito, T.Murakami, H.Sasano, N.Yaegashi, K.Okamura
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Journal Title
Am J Reprod Immunol 60
Pages: 534-540
Peer Reviewed
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[Journal Article] Hematopoietic stem cell-engrafted NOD/SCID/IL2Rgamma null mice develop human lymphoid systems and induce long-lasting HIV-1 infection with specific humoral immune responses.2007
Author(s)
Watanabe S., K.Terashima, S.Ohta, S.Horibata, M.Yajima, Y.Shiozawa, M.Z.Dewan, Z.Yu, M.Ito, T.Morio, N.Shimizu, M.Honda, N.Yamamoto
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Journal Title
Peer Reviewed
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[Journal Article] Human cord blood CD34+ cells develop into hepatocytes in the livers of NOD/SCID/gamma(c)null mice through cell fusion.2007
Author(s)
Fujino H., H.Hiramatsu, A.Tsuchiya, A.Niwa, H.Noma, M.Shiota, K.Umeda, M.Yoshimoto, M.Ito, T.Heike, T.Nakahata
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Journal Title
Faseb J 21
Pages: 3499-3510
Peer Reviewed
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[Journal Article] Clonal analysis of thymus-repopulating cells presents direct evidence for self-renewal division of human hematopoietic stem cells.2006
Author(s)
Yahata T., S.Yumino, Y.Seng, H.Miyatake, T.Uno, Y.Muguruma, M.Ito, H.Miyoshi, S.Kato, T.Hotta, K.Ando
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Journal Title
Blood 108
Pages: 2446-2454
Peer Reviewed
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[Journal Article] Development of human-human hybridoma from anti-Her-2 peptide-producing B cells in immunized NOG mouse.2006
Author(s)
Kametani Y., M.Shiina, I.Katano, R.Ito, K.Ando, K.Toyama, H.Tsukamoto, T.Matsumura, Y.Saito, D.Ishikawa, T.Taki, M.Ito, K.Imai, Y.Tokuda, S.Kato, N.Tamaoki, S.Habu
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Journal Title
Exp Hematol 34
Pages: 1239-1247
Peer Reviewed
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