Structures and Functions of Membrane-Bound Biomolecules

2010 Fiscal Year Final Research Report

• Project/Area Number: 18101010
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Osaka University
• Principal Investigator: MURATA Michio Osaka University, 大学院・理学研究科, 教授 (40183652)
• Co-Investigator(Kenkyū-buntansha): OISHI Tohru 九州大学, 大学院・理学研究院, 教授 (90241520) ; MATSUMORI Nobujaki 大阪大学, 大学院・理学研究科, 准教授 (50314357) ; DOI Mototsugu 大阪大学, 大学院・理学研究科, 技術補佐員 (10379307)
• Project Period (FY): 2006 – 2010
• Keywords: 脂質二重膜 / 抗生物質 / 脂質マイクロドメイン / 分子複合体自己会合体 / コレステロール / 膜タンパク質

Research Abstract

Biomembrane, essential entity for life, has been regarded as an elusive liquid medium. In this project, we have examined the 3D structures of membrane lipids which are responsible for biological functions. Solid-state NMR measurements for isotope-labeled molecules allowed us to elucidate the structures and dynamics of antibiotics and phospholipids residing in bilayer membranes.

Research Products

• [Journal Article] Synthesis of 6-F-ergosterol and its influence on membrane-permeabilization of amphotericin B and amphidinol 3. (2011)
  • Author(s): Kasai, Y., Matsumori, N., Ueno, H., Nonomura, K., Yano, S. Murata, M., Oishi, T.
  • Journal Title: Org.Biomol.Chem. 9 Pages: 1437-1442
  • Peer Reviewed
• [Journal Article] Conformations of spermine in ATP complex : The structural basis for weak bimolecular interactions of major cellular electrolytes. (2011)
  • Author(s): Maruyoshi, M., Yamaguchi, T., Demura, T., Matsumori, N., Oishi, T., Murata, M.
  • Journal Title: Chem.Eur.J. 17 Pages: 4788-4795
  • Peer Reviewed
• [Journal Article] effect on interaction between amphidinol 3 and liposomal membrane as evidenced by surface plasmon resonance. (2010)
  • Author(s): Swasono, R., Mouri, R., Morsy, N., Matsumori, N., Oishi, T., Murata, M.Sterol
  • Journal Title: Bioorg.Med.Chem.Lett. 20 Pages: 2215-2218
  • Peer Reviewed
• [Journal Article] Identification of Rap1A as yessotoxin binding target from blood cell membranes. (2010)
  • Author(s): Ujihara, S., Oishi, T., Mouri, R., Tamate, R., Konoki, K., Matsumori, N., Murata, M., Oshima, Y., Sugiyama, N., Tomita, M., Ishihama, Y.
  • Journal Title: Bioorg.Med.Chem.Lett. 20 Pages: 6443-6446
  • Peer Reviewed
• [Journal Article] Conformational change of spermidine upon interacting with ATP in aqueous solution. (2009)
  • Author(s): Maruyoshi, K., Nonaka, K., Sagane, T., Demura, T., Yamaguchi, T., Matsumori, N., Oishi, T., Murata, M
  • Journal Title: Chem.Eur.J. 15 Pages: 1618-1626
  • Peer Reviewed
• [Journal Article] interaction between amphotericin B and ergosterol in lipid bilayers as revealed by 2H (2009)
  • Author(s): Matsumori, N., Tahara, K., Yamamoto, H., Morooka, A., Doi, M., Oishi, T., Murata, M.Direct
  • Journal Title: NMR.J.Am.Chem.Soc. 131 Pages: 11855-11860
  • Peer Reviewed
• [Journal Article] Surface resonance-based detection of ladder-shaped polyether by competitive binding method. (2009)
  • Author(s): Mouri, R., Oishi, T., Torikai, K., Ujihara, S., Matsumori, N., Murata, M., Oshima, Y.
  • Journal Title: Bioorg.Med.Chem.Lett. 19 Pages: 2824-2828
  • Peer Reviewed
• [Journal Article] Amphotericin B-induced ion flux is markedly attenuated in phosphatidylglycerol membrane as evidenced by a newly devised fluorometric method. (2009)
  • Author(s): Takano, T., Konoki, K., Matsumori, N., Murata, M.
  • Journal Title: Bioorg.Med.Chem. 17 Pages: 6301-6304
  • Peer Reviewed
• [Journal Article] Self-assembly of amphotericin B is probably surrounded by ergosterol; bimolecular interactions as evidenced by solid stata NMR and CD spectra. (2008)
  • Author(s): Kasai, Y., Matsumori, N., Umegawa, Y., Matsuoka, S., Ueno, H., Ikeuchi, H., Oishi, T., Murata, M.
  • Journal Title: Chem.Eur.J. 14 Pages: 1178-1185
  • Peer Reviewed
• [Journal Article] Effects of lipid constituents on membrane-permeabilizing activity of amphidinols. (2008)
  • Author(s): Morsy, N., Houdai, T., Konoki, K., Matsumori, N., Oishi, T., Murata, M
  • Journal Title: Bioorg.Med.Chem. 16 Pages: 3084-3090
  • Peer Reviewed
• [Journal Article] Roles of integral protein in membrane permeabilization by amphidinols. (2008)
  • Author(s): Morsy, N., Konoki, K., Houdai, T., Matsumori, N.Oishi, T., Murata, M., Aimoto, S.
  • Journal Title: Biochim.Biophys.Acta. 1778 Pages: 1453-1459
  • Peer Reviewed
• [Journal Article] Orientation of fluorinated cholesterol in lipid bilayers analyzed by 19F tensor calculation and solid-state NMR. (2008)
  • Author(s): Matsumori, N., Kasai, Y., Oishi, T., Murata, M., Nomura, K.
  • Journal Title: J.Am.Chem.Soc. 130 Pages: 4757-4766
  • Peer Reviewed
• [Journal Article] Complex formation of amphotericin B in sterol-containing membrane as evidenced by surface plasmon resonance. (2008)
  • Author(s): Mouri, R., Konoki, K., Matsumori, N., Oishi, T., Murata, M.
  • Journal Title: Biochemistry 47 Pages: 7807-7815
  • Peer Reviewed
• [Journal Article] Design and synthesis of ladder-shaped tetracyclic, heptacyclic, and decacyclic ethers and evaluation of the interaction with transmembrane proteins. (2008)
  • Author(s): Torikai, K., Oishi, T., Ujihara, S., Matsumori,N., Koniki, K.Murata, M., Aimoto, S.
  • Journal Title: J.Am.Chem.Soc. 30 Pages: 10217-10226
  • Peer Reviewed
• [Journal Article] Structure of membrane-bound amphidinol 3 in isotropic small bicelles. (2008)
  • Author(s): Houdai, T., Matsumori, N., Murata, M.
  • Journal Title: Org.Lett. 10 Pages: 4191-4194
  • Peer Reviewed
• [Journal Article] Interaction of ladder-shaped polyethers with transmembrane α-helix of glycophorin A as evidenced by saturation transfer difference NMR and surface plasmon resonace. (2008)
  • Author(s): Ujihara, S., Oishi, T., Torikai, K., Konoki, K., Matsumori, N., Murata, M., Oshima, Y., Aimoto, S.
  • Journal Title: Bioorg.Med.Chem.Lett. 18 Pages: 6115-6118
  • Peer Reviewed
• [Journal Article] Ergosterol increases intermolecular distance of amphotericin B in membrane-bound assembly as evidenced by solid-state NMR. (2008)
  • Author(s): Umegawa, Y., Matsumori, N., Oishi, T.,Murata, M.
  • Journal Title: Biochemistry 47 Pages: 13463-13469
  • Peer Reviewed
• [Journal Article] Conformation and position of membrane-bound amphotericin B deduced from NMR in SDS micelles. (2007)
  • Author(s): Matsumori, N., Houdai, T., Murata, M.
  • Journal Title: J.Org.Chem. 72 Pages: 700-704
  • Peer Reviewed
• [Journal Article] Conformation and location of membrane-bound salinomycin-sodium complex deduced from NMR in isotropic bicelles. (2007)
  • Author(s): Matsumori, N.Morooka, A., Murata, M.
  • Journal Title: J.Am.Chem.Soc. 129 Pages: 14889-14995
  • Peer Reviewed
• [Journal Article] Detailed description on Conformation and Location of Membrane-bound Erythromycin A using Small Bicelle. (2006)
  • Author(s): Matsumori, N., Morooka, A., Murata, M.
  • Journal Title: J.Med.Chem. 49 Pages: 3501-3508
  • Peer Reviewed
• [Journal Article] Membrane interaction of amphotericin B as single-Length assembly examined by solid state NMR for uniformly ^<13>C-enriched Agent. (2006)
  • Author(s): Matsuoka, S., Ikeuchi, H., Umegawa, Y., Matsumori, N., Murata, M.
  • Journal Title: Bioorg.Med.Chem. 14 Pages: 6608-6614
  • Peer Reviewed
• [Journal Article] Synthesis of 28-19F-amphotericn B methyl ester. (2006)
  • Author(s): Tsuchikawa, H., Matsushita, N., Matsumori, N., Murata, M., Oishi, T.
  • Journal Title: Tetrahedron Lett. 47 Pages: 6187-6191
  • Peer Reviewed
• [Presentation] Molecular Assemblies in Biomembranes as Viewed by Solid State NMR (2008)
  • Author(s): Michio Murata
  • Organizer: Naito Conference Chemical Biology(シャトレーゼガトーキングダム)
  • Place of Presentation: 札幌(招待講演)
  • Year and Date: 20080909-20080911
• [Presentation] Synthesis of fluorine labeled natural products for solid-state NMR measurements (2008)
  • Author(s): Tohru Oishi
  • Organizer: 236th ACS National Meeting
  • Place of Presentation: Philadelphia, USA(招待講演)
  • Year and Date: 20080817-20080821
• [Presentation] Natural products forming functional assemblies in biomembrane (2008)
  • Author(s): Michio Murata
  • Organizer: Mona Symposium
  • Place of Presentation: Kingston, JAMAICA(招待講演)
  • Year and Date: 20080107-20080109
• [Presentation] Ion channel complex of antibiotics as viewed by NMR (2008)
  • Author(s): Michio Murata
  • Organizer: PAC ICOB-6, SCNP-26
  • Place of Presentation: Charlottetown, CANADA(招待講演)
  • Year and Date: 2008-07-17
• [Presentation] Funcitonal Membrane Complexes (2008)
  • Author(s): Steroids as a Key Player in Assembling Michio Murata
  • Organizer: Nakanishi Symposium
  • Place of Presentation: 立教大学(招待講演)
  • Year and Date: 2008-03-28
• [Presentation] Yessotoxin and synthetic polyether models-Interactions with peptides in membrane (2007)
  • Author(s): Michio Murata
  • Organizer: Vth European Conference on Marine Natura Products
  • Place of Presentation: Ischia, ITALY(招待講演)
  • Year and Date: 20070916-20070921
• [Presentation] Marine cyclic polyethers-their interaction with membrane proteins (2007)
  • Author(s): Michio Murata
  • Organizer: 日米セミナー海洋天然物
  • Place of Presentation: Salt Lake City, USA(招待講演)
  • Year and Date: 20070701-20070707
• [Presentation] Synthetic studies of yessotoxin (2007)
  • Author(s): Tohru Oishi
  • Organizer: International Symposium on Catalysts and Fine Chemicals
  • Place of Presentation: Nanyang Tech.Univ.Singapore(招待講演)
  • Year and Date: 2007-12-17
• [Presentation] Synthetic studies of natural and artificial ladder-shaped polyethers for exploring biological functions (2006)
  • Author(s): Tohru Oishi
  • Organizer: Sendai Postsymposium of ICOB-5, ISCNP-25
  • Place of Presentation: 仙台国際センター(招待講演)
  • Year and Date: 2006-07-30
• [Remarks] ホームページ等
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/murata/