Study on gibberellin perception

2010 Fiscal Year Final Research Report

• Project/Area Number: 18107001
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: Nagoya University
• Principal Investigator: MATSUOKA Makoto Nagoya University, 生物機能開発利用研究センター, 教授 (00270992)
• Co-Investigator(Kenkyū-buntansha): NAKOJIMA Masatosi 東京大学, 農学生命科学研究科, 助教 (50237278) ; HATTORI Tsukaho 名古屋大学, 生物機能開発利用研究センター, 教授 (10164865)
• Project Period (FY): 2006 – 2010
• Keywords: 成長生理 / 植物ホルモン

Research Abstract

We analyzed the 3D structure of rice GA receptor (OsGID1) bound with GA. The overall structure shows an α/β-hydrolase fold similar to that of HSLs. The GA-binding pocket corresponds to the substrate-binding site of HSLs. On the basis of the OsGID1 structure, we mutagenized important residues for GA binding and examined their binding activities. Almost all of them showed very little or no activity, confirming that the residues revealed by structural analysis are important for GA binding. The observations indicate that GID1 originated from HSL and was further modified to have higher affinity and more strict selectivity for bioactive GAs by adapting the amino acids involved in GA binding in the course of plant evolution.

Research Products

• [Journal Article] Characterization of the molecular mechanism underlying gibberellin perception complex formation in rice. (2010)
  • Author(s): Hirano, K., Asano, K., Tsuji, H., Kawamura, M., Mori, H., Kitano, H., Ueguchi-Tanaka, M., Matsuoka M.
  • Journal Title: Plant Cell 22,(8) Pages: 2680-2696
  • Peer Reviewed
• [Journal Article] A rice gidl suppressor mutant reveals that gibberellin is not always required for interaction between its receptor, GID1, and DELLA proteins. (2010)
  • Author(s): Yamamoto, Y., Hirai, T., Yamamoto, E., Kawamura, M., Sato, T., Kitano, H., Matsuoka, M., Ueguchi-Tanaka, M.
  • Journal Title: Plant Cell 22,(11) Pages: 3589-3602
  • Peer Reviewed
• [Journal Article] The perception of gibberellins : clues from receptor structure. (2010)
  • Author(s): Ueguchi-Tanaka, M., Matsuoka, M.
  • Journal Title: Curr.Opin.Plant Biol. 13,(5) Pages: 503-508.
  • Peer Reviewed
• [Journal Article] Isolation and characterization of dominant dwarf mutants, Slr1-d, in rice. (2009)
  • Author(s): Asano, K., Hirano, K., Ueguchi-Tanaka, M., Angeles-Shim, R.B., Komura, T., Satoh, H., Kitano, H., Matsuoka, M., Ashikari, M.
  • Journal Title: Mol.Genet.Genomics 281,(2) Pages: 223-231.
  • Peer Reviewed
• [Journal Article] Bar the windows : an optimized strategy to survive drought and salt adversities. (2009)
  • Author(s): Song X, Matsuoka M.
  • Journal Title: Genes Dev. 1;23(15) Pages: 1709-1713
• [Journal Article] Differential expression and affinities of Arabidopsis gibberellin receptors can explain variation in phenotypes of multiple knock-out mutants. (2009)
  • Author(s): Suzuki, H., Park, S.H., Okubo, K., Kitamura, J., Ueguchi-Tanaka, M., Iuchi, S., Katoh, E., Kobayashi, M., Yamaguchi, I., Matsuoka, M., Asami, T., Nakajima M.
  • Journal Title: Plant J. 60,(1) Pages: 48-55
  • Peer Reviewed
• [Journal Article] Functional analysis of cotton orthologs of GA signal transduction factors GID1 and SLR1. (2008)
  • Author(s): Aleman, L., Kitamura, J., Abdel-Mageed, H., Lee, J., Sun, Y., Nakajima, M., Ueguchi-Tanaka, M., Matsuoka, M., Allen, RD.
  • Journal Title: Plant Mol.Biol. 68,(1-2) Pages: 1-16
  • Peer Reviewed
• [Journal Article] Release of the Repressive Activity of Rice DELLA Protein SLR1 by Gibberellin Does Not Require SLR1 Degradation in the gid2 Mutant. (2008)
  • Author(s): Ueguchi-Tanaka, M., Hirano, K., Hasegawa, Y., Kitano, H., Matsuoka, M.
  • Journal Title: Plant Cell 20,(9) Pages: 2437-2446
  • Peer Reviewed
• [Journal Article] Structural basis for gibberellin recognition by its receptor GID1. (2008)
  • Author(s): Shimada, A., Ueguchi-Tanaka, M., Nakatsu, T., Nakajima, M., Naoe, Y., Ohmiya, H., Kato, H., Matsuoka, M.
  • Journal Title: Nature 456,(7221) Pages: 520-523
  • Peer Reviewed
• [Journal Article] Multiple loss-function of Arabidopsis gibberellin receptor AtGID1s completely shuts down a gibberellin signal. (2007)
  • Author(s): Iuchi, S., Suzuki, H., Kim, Y.-C., Iuchi, A., Kuromori, T., Ueguchi-Tanaka, M., Asami, T., Yamaguchi, I., Matsuoka, M., Kobayashi, M., Nakajima, M.
  • Journal Title: Plant J. 50,(6) Pages: 958-966
  • Peer Reviewed
• [Journal Article] Molecular interactions of a soluble gibberellin receptor, GID1, with a rice DELLA protein, SLR1, and gibberellin. (2007)
  • Author(s): Ueguchi-Tanaka, M., Nakajima, M., Katoh, E., Ohmiya, H., Asano, K., Saji, S., Hongyu, X., Ashikari, M., Kitano, H., Yamaguchi, I., Matsuoka, M.
  • Journal Title: Plant Cell 19,(7) Pages: 2140-2155
  • Peer Reviewed
• [Journal Article] Identification and characterization of Arabidopsis gibberellin rexeptor. (2006)
  • Author(s): Nakajima M., Shimada, A., Takashi, Y., Kim, Y-C., Park, S-H., Ueguchi-Tanaka, M., Suzuki, H., Katoh, E., Iuchi, S., Kobayashi, M., Maeda, T., Matsuoka, M., Yamaguchi, I.
  • Journal Title: Plant J. 46,(5) Pages: 880-889
  • Peer Reviewed
• [Journal Article] The rice SPINDLY gene functions as a negat ive regulator of gibberellin signaling by controlling the suppressive function of the DELLA protein, SLR1, and modulating brassinosteroid synthesis. (2006)
  • Author(s): Shimoda, A., Ueguchi-Tanaka, M., Sakamoto, T., Fujioka, S., Takatsuto, S., Yoshida, S., Sazuka, T., Ashikari, M., Matsuoka, M.
  • Journal Title: Plant J. 48,(3) Pages: 390-402
  • Peer Reviewed
• [Journal Article] A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos.
  • Author(s): Sazuka, T., Kamiya, N., Nishimura, T., Ohmae, K., Sato, Y., Tmamura, K., Nagato, Y., Koshiba, T., Nagamura, Y., Ashikari, M., Kitano, H., Matsuoka M.
  • Journal Title: Plant J.
  • Peer Reviewed
• [Presentation] ジベレリン変異体における倒伏抵抗性の評価 (2011)
  • Author(s): 奥野綾子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台(要旨集)
  • Year and Date: 20110300
• [Presentation] Molecular Interaction of Gibberellin Signaling Components, GID1, SLR1, and GID2. (2010)
  • Author(s): Matsuoka, M.
  • Organizer: The 21st International Conference on Arabidopsis Research(ICAR 2010).
  • Place of Presentation: Yokohama, JAPAN(招待講演)
  • Year and Date: 2010-06-07
• [Presentation] ジベレリン受容体のシグナル分担制御機構の解析 (2009)
  • Author(s): Seung-Hyun Park
  • Organizer: 植物化学調節学会
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-29
• [Presentation] Structural basis for gibberellin recognition by its receptor GTD1. (2008)
  • Author(s): Matsuoka, M.
  • Organizer: The 6th international Symposium of Rice Functional Genomics.
  • Place of Presentation: Jeju, Korea
  • Year and Date: 20081110-20081112
• [Presentation] Tharacterization of Arabidopsis gibberellin receptors (2007)
  • Author(s): Nakajima, M.
  • Organizer: 19th Tnternatinal Conference on Plant Growth Substances
  • Place of Presentation: Puerto Vallarta, Mexico
  • Year and Date: 20070722-20070723
• [Presentation] Gibberellin porception and signal transduction in rice. (2007)
  • Author(s): Ueguchi-Tanaka, M.
  • Organizer: 19th TPGSA Meeting.
  • Place of Presentation: Puerto Vallarta, Mexico
  • Year and Date: 20070722-20070723