Molecular mechanism underlying metameric morphogenesis

2010 Fiscal Year Final Research Report

• Project/Area Number: 18107006
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2010); National Institutes of Natural Sciences Okazaki Research Facilities (2006-2009)
• Principal Investigator: TAKADA Shinji 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (60206753)
• Co-Investigator(Kenkyū-buntansha): KOSHIDA Sumito 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (40342638) ; OHKUBO Tadashi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (10450719)
• Co-Investigator(Renkei-kenkyūsha): YABE Taijiro 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (30470074) ; AKANUMA Takashi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 研究員 (50450721) ; KAWAMURA Akinori 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 学振特別研究員 (10466691) ; TAKADA Ritsuko 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 研究員 (40450720)
• Research Collaborator: UTSUMI Hideko 基礎生物学研究所, 分子発生学研究部門, 技術職員 ; CHEN Quihong 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 研究員 ; TAKAHASHI Jun 総合研究大学院大学, 大学院生 ; TSUNOKUNI Hiroyuki 総合研究大学院大学, 大学院生 ; WANGLAR Chimwar 総合研究大学院大学, 大学院生
• Project Period (FY): 2006 – 2010
• Keywords: 発生・分化 / 細胞・組織 / 遺伝子 / 発現制御

Research Abstract

To understand the molecular mechanism underlying the development of metameric structures, we identified several genes required for somite segmentation. One of these genes, Ripply1, represses the function of T-box transcription factors through several ways, resulting in proper positioning of the segmentation boundary. Furthermore, the interaction between Ripply and T-box factors also appears to be required for the segmentation of another metameric structure, the pharyngeal arch, suggesting that a common machinery is involved in segmentation of different tissues.

Research Products

• [Journal Article] Ripply3, a Tbx1 repressor, is required for development of the pharyngeal apparatus and its derivatives in mice (2011)
  • Author(s): Okubo, T., Kawamura, A., Takahashi, J., Yagi, H., Morishima, M., Matsuoka, R., & Takada, S.
  • Journal Title: Development Volume: 138 Pages: 339-348
  • Peer Reviewed
• [Journal Article] Analysis of Ripply1/2-deficient mouse embryos reveals a mechanism underlying the rostro-caudal patterning within a somite (2010)
  • Author(s): Takahashi J., Ohbayashi A., Oginuma M., Saito D., Mochizuki A., Saga Y., & Takada S.
  • Journal Title: Dev. Biol Volume: 342 Pages: 134-145
  • Peer Reviewed
• [Journal Article] Planar polarization of node cells determines the rotational axis of node cilia (2010)
  • Author(s): Hashimoto, H., Shinohara, K., Wang, J., Ikeuchi. S., Yoshiba, S., Meno, C., Nonaka, S., Takada, S., Hatta, K., Wynshaw-boris, A., & Hamada, H.
  • Journal Title: Nat Cell Biol Volume: 12 Pages: 170-176
  • Peer Reviewed
• [Journal Article] A novel regulatory mechanism for Fgf18 signaling involving cysteine-rich FGF receptor(Cfr) and delta-like protein(Dlk) (2010)
  • Author(s): Miyaoka, Y., Tanaka, M., Imamura, T., Takada, S., & Miyajima, A.
  • Journal Title: Development Volume: 137 Pages: 159-167
  • Peer Reviewed
• [Journal Article] Activator-to-repressor conversion of T-box transcription factors by the Ripply family of Groucho/TLE-associated mediators (2008)
  • Author(s): Kawamura, A., Koshida, S., & Takada, S.
  • Journal Title: Mol. Cell Biol Volume: 28 Pages: 3236-3244
  • Peer Reviewed
• [Journal Article] Wnt canonical pathway restricts graded Shh/Gli patterning activity through the regulation of Gli3 expression (2008)
  • Author(s): Alvarez-Medina, R., Cayuso, J., Okubo, T., Takada, S., & Marti, E.
  • Journal Title: Development Volume: 135 Pages: 237-247
  • Peer Reviewed
• [Journal Article] Paf1 complex homologes are required for Notch-regulated transcription during somite segmentation (2007)
  • Author(s): Akanuma, T., Koshida, S., Kawamura, A., Kishimoto, Y., & Takada, S.
  • Journal Title: EMBO Rep Volume: 8 Pages: 858-863
  • Peer Reviewed
• [Journal Article] Monounsaturated fatty acid modification of Wnt proteins : Its role in Wnt secretion (2006)
  • Author(s): Takada, R., Satomi, Y., Kurata, T., Ueno, N., Norioka, S., Kondoh, H., Takao, T., & Takada, S.
  • Journal Title: Dev. Cell Volume: 11 Pages: 791-801
  • Peer Reviewed
• [Journal Article] Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse (2006)
  • Author(s): Yamaguchi, Y., Yonemura, S., & Takada, S.
  • Journal Title: Development Volume: 133 Pages: 4737-4748
  • Peer Reviewed
• [Presentation] Jagged/Notch is required for epithelial organization and maintenance of assembled fibronectin during pharyngeal morphogenesis, in Exiting Biologies : Biology at the interface (2011)
  • Author(s): Takada S
  • Place of Presentation: RIKEN CDB, Kobe
  • Year and Date: 2011-09-29
• [Presentation] ゼブラフィッシュの咽頭嚢形成における上皮組織の再組織化 (2011)
  • Author(s): 高田慎治
  • Organizer: 第84回日本生化学会(シンポジウム)
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2011-09-22
• [Presentation] Jagged/Notch regulates epithelial morphogenesis during the development of pharyngeal pouch (2011)
  • Author(s): Takada S
  • Organizer: The 5th Asia-Oceania Zebrafish Meeting
  • Place of Presentation: Beijing
  • Year and Date: 2011-08-27
• [Presentation] 咽頭弓と体節の発生におけるJaggedシグナルとフィブロネクチンの相互作用 (2010)
  • Author(s): 高田慎治
  • Organizer: 第62回日本細胞生物学会(シンポジウム)
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-05-19
• [Presentation] Tbx-associated transcriptional corepressor Ripply3 is essential for pharyngeal arch development (2008)
  • Author(s): Okubo, T. Kawamura, A., Takahashi, J., Ohbayashi, A., & Takada, S.
  • Organizer: 第31回日本分子生物学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2008-12-09
• [Presentation] Ripply3, a negative regulator of Tbx1, is required for development of caudal pharyngeal arches and their derivatives (2008)
  • Author(s): Takada S
  • Organizer: Cold Spring Harbor Meeting on Mouse Molecular Genetics
  • Place of Presentation: Cold Spring Harbor(USA)
  • Year and Date: 2008-10-31
• [Presentation] Transcriptional repression of mesp gene via Groucho/TLE-associated mediator Ripply (2007)
  • Author(s): Takada S
  • Organizer: Satellite meeting to the 66^<th> Annual Meeting of the Society for Developmental Biology and the First Pan-American Congress in Developmental Biology : Somitogenesis : from models to Therapeutics
  • Place of Presentation: Cancun(Mexico)
  • Year and Date: 2007-06-16
• [Book] 咽頭弓からの胸腺形成と先天性異常の発症機構 (2012)
  • Author(s): 大久保直、高田慎治
  • Total Pages: 168-176
• [Book] Groucho結合因子Ripply1による分節プログラムの制御 (2006)
  • Author(s): 川村哲規、越田澄人、高田慎治
  • Total Pages: 272-273
• [Remarks]
  • URL: http://www.nibb.ac.jp/cib2/