Molecular mechanisms of biosynthesis and exudation of strigolactones and their manipulation for regulation of parasitism and symbiosis

2009 Fiscal Year Final Research Report

• Project/Area Number: 18208010
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Utsunomiya University
• Principal Investigator: YONEYAMA Koichi Utsunomiya University, 雑草科学研究センター, 教授 (00114174)
• Co-Investigator(Kenkyū-buntansha): TAKEUCHI Yasutomo 宇都宮大学, 雑草科学研究センター, 名誉教授 (90008003) ; SEKIMOTO Hitoshi 宇都宮大学, 農学部, 教授 (10261819) ; YOKOTA Takao 帝京大学, 理工学部, 教授 (40011986) ; SUGIMOTO Yukihiro 神戸大学, 農学部, 教授 (10243411) ; AKIYAMA Kohki 大阪府立大学, 生命環境科学研究科, 准教授 (20285307) ; NOMURA Takahito 宇都宮大学, 雑草科学研究センター, 准教授 (60373346)
• Project Period (FY): 2006 – 2009
• Keywords: 根寄生雑草 / アーバスキュラー菌根菌 / ストリゴラクトン / 発芽刺激物質 / 菌糸分岐誘導物質 / 枝分かれ / 植物ホルモン / カロテノイド

Research Abstract

Strigolactones exuded from plant roots elicit seed germination of root parasitic plants and induce hyphal branching of symbiotic arbuscular mycorrhizal (AM) fungi. Therefore, strigolactones function as host recognition signals for both parasitism and symbiosis. Studies were conducted to understand molecular mechanisms of strigolactone biosynthesis and exudation. Through the studies, 1) several novel strigolactones were isolated and their structures determined, 2) effects of nutrients on strigolactone production and exudation were clarified, 3) structure-activity relationships of strigolactones in germination stimulation of root parasitic weed seeds and in induction of hyphal branching of AM fungi were elucidated, and 4) interactions between root parasitic plants with their compatible and incompatible host plants were examined. In addition, excessive branching mutants of rice plants were found to be the deficit either in biosynthesis or signal transduction of strigolactones, demonstrating that strigolactones are a novel class of plant hormones inhibiting shoot branching.

Research Products

• [Journal Article] Reactions of Lotus japonicus ecotypes and mutants to root parasitic plants (2009)
  • Author(s): Kubo, M., Ueda, H., Park, P., Kawaguchi, M., Sugimoto, Y.
  • Journal Title: J.Plant Physiol. 166 Pages: 353-362
• [Journal Article] Strigolactones ; structures and biological activities (2009)
  • Author(s): Yoneyama, K., Xie, X., Yoneyama, K., Takeuchi, Y.
  • Journal Title: Pest Manag.Sci. 65 Pages: 467-470
• [Journal Article] Strigolactones, host recognition signals for root parasitic plants and arbuscular mycorrhizal fungi, from Fabaceae plants (2008)
  • Author(s): Yoneyama, K., Xie, X., Sekimoto, H., Takeuchi, Y., Ogasawara, S., Akiyama, K., Hayashi, H., Yoneyama, K.
  • Journal Title: New Phytol. 179 Pages: 484-494
• [Journal Article] Inhibition of shoot branching by new terpenoid plant hormones (2008)
  • Author(s): Umehara, M., Hanada, A., Yoshida, S., Akiyama, K., Arite, T., Takeda-Kamiya, N., Magome, H., Kamiya, Y., Shirasu, K., Yoneyama, K., Kyozuka, J., Yamaguchi, S.
  • Journal Title: Nature 455 Pages: 195-200
• [Journal Article] Sorgomol, germination stimulant for root parasitic plants, produced by Sorghum bicolor (2008)
  • Author(s): Xie, X., Yoneyama, K., Kusumoto, D., Yamada, Y., Takeuchi, Y., Sugimoto, Y., Yoneyama, K.
  • Journal Title: Tetrahedron Lett. 49 Pages: 2066-2068
• [Journal Article] Nitrogen deficiency as well as phosphorus deficiency in sorghum promotes the production and exudation of 5-deoxystrigol, the host recognition signal for arbuscular mycorrhizal fungi and root parasites (2007)
  • Author(s): Yoneyama, K., Xie, X., Kusumoto, D., Sekimoto, H., Sugimoto, Y., Takeuchi, Y., Yoneyama, K.
  • Journal Title: Planta 227 Pages: 125-132
• [Journal Article] Structure-activity relationship of naturally occurring strigolactones in Orobanche minor seed germination stimulation
  • Author(s): Kim, H. I., Xie, X., Kim, H. S., Chun, J. C., Yoneyama, K., Nomura, T., Takeuchi, Y., Yoneyama, K.
  • Journal Title: J.Pestic.Sci. (in press)
• [Journal Article] Strigolactones as germination stimulants for root parasitic plants
  • Author(s): Yoneyama, K., Awad, A. A., Xie, X., Yoneyama, K., Takeuchi, Y.
  • Journal Title: Plant Cell Physiol. (in press)
• [Presentation] 新規ストリゴラクトン fabacol の単離および構造決定 (2010)
  • Author(s): 謝肖男, 原田裕大, 竹内安智, 米山弘一
  • Organizer: 日本農芸化学会
  • Year and Date: 20100300
• [Presentation] Strigolactones as a new plant growth regulator (2009)
  • Author(s): Yoneyama K, Xie X, Yoneyama K, Takeuchi Y.
  • Organizer: MARCO Symposium 2009, Challenges for Agro-Environmental Research in Monsoon Asia
  • Place of Presentation: Tsukuba
  • Year and Date: 20091005-20091007
• [Presentation] Qualitative and quantitative differences in strigolactone exudation between Striga tolerant and susceptible maize cultivars (2009)
  • Author(s): Yoneyama K, Takeuchi Y, Yokota T, Kanampiu F, Yoneyama K, Nomura T.
  • Organizer: 10^<th> World Congress on Parasitic Plants
  • Place of Presentation: Kusadasi, Turkey
  • Year and Date: 20090608-20090612
• [Presentation] Strigolactones, structure, activity, and regulation of production (2008)
  • Author(s): Yoneyama K, Xie X, Yoneyama K, Takeuchi Y.
  • Organizer: OECD-CRP : Managing Parasitic Weeds : Integrating Science and Practice. OECD-CRP : Biological Resource Management for Sustainable Agricultural Systems
  • Place of Presentation: Ostuni, Italy
  • Year and Date: 20080921-20080926
• [Presentation] Characterization of strigolactones, plant derived signals for symbiosis and parasitism (2008)
  • Author(s): Yoneyama K, Xie X, Yoneyama K, Takeuchi Y.
  • Organizer: 5^<th> International Weed Science Congress
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 20080623-20080627
• [Book] Comprehensive Natural Products II. Chemistry and Biology (2010)
  • Author(s): Yoneyama, K., Natsume, M.
  • Total Pages: 539-561
  • Publisher: Elsevier
• [Book] ストリゴラクトンを利用する根寄生植物 植物のシグナル伝達 分子と応答 (2009)
  • Author(s): 米山香織, 謝肖男, 米山弘一
  • Total Pages: 165-171
  • Publisher: 共立出版
• [Book] 第5章 発芽と土壌中化学物質「発芽生物学」 (2009)
  • Author(s): 米山弘一, 米山香織
  • Total Pages: 105-122
• [Remarks] ホームページ
  • URL: Http://www.wsc.mine.utsunomiya-u.ac.jp