Mechanisms of Sensory Processing in C.elegans Neuronal Circuits.

2009 Fiscal Year Final Research Report

• Project/Area Number: 18370004
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Kyushu University
• Principal Investigator: ISHIHARA Takeshi Kyushu University, 大学院・理学研究院, 教授 (10249948)
• Co-Investigator(Kenkyū-buntansha): KOGA Makoto 九州大学, 大学院・理学研究院, 准教授 (60243888) ; FUJIWARA Manabi 九州大学, 大学院・理学研究院, 助教 (70359933) ; HIROTSU Takaaki 九州大学, 大学院・理学研究院, 助教 (70404035)
• Project Period (FY): 2006 – 2009
• Keywords: 行動遺伝学 / 学習 / 神経回路 / 情報処理 / 線虫

Research Abstract

Animals sense variety of environmental stimuli in sensory neurons, simultaneously. This sensory information is processed in central nervous systems to regulate their behavior. In this study, we investigated mechanisms of the sensory processing by using C.elegans as a model organism. We identified signaling pathways to regulate the sensory integration and neuronal circuits where those pathways function. To elucidate the regulation of the memory retention, we isolated mutants showing longer memory retention and thereby identified a signaling pathway that regulates the memory retention. We found that the germline signals regulate the olfactory response. By using this phenomenon, we isolated a mutant defective in the regulation. In addition, we elucidated the function of signaling pathways for the olfactory sensation and behavioral plasticity.

Research Products

• [Journal Article] Behavioural assay for olfactory plasticity in C. elegans. (2009)
  • Author(s): Hirotsu T., Hayashi Y., Iwata R., Kunitomo H., Kage-Nakadai E., Kubo T., Ishihara T., Iino Y.
  • Journal Title: Nature Protocols 139
• [Journal Article] A trophic role for Wnt-Ror kinase signaling during developmental pruning in Caenorhabditis elegans. (2009)
  • Author(s): Hayashi Y., Hirotsu T., Iwata R., Kage-Nakadai E., Kunitomo H., Ishihara T., Iino Y., Kubo T.
  • Journal Title: Nature Neuroscience 12 Pages: 981-987
  • Peer Reviewed
• [Journal Article] FLR-2, the glycoprotein hormone alpha subunit, is involved in the neural control of intestinal functions in Caenorhabditis elegans. (2009)
  • Author(s): Oishi A., Gengyo-Ando K., Mitani S., Mohri-Shiomi A., Kimura K.D., Ishihara T., Katsura I.
  • Journal Title: Genes to Cells 14 Pages: 1141-54
  • Peer Reviewed
• [Journal Article] ADBP-1 regulates an ADAR RNA-editing enzyme to antagonize RNAi-mediated gene silencing in C. elegans. (2008)
  • Author(s): Ohta, H., Fujiwara, M., Ohshima, Y., Ishihara
  • Journal Title: T. Genetics 180 Pages: 785-796
  • Peer Reviewed
• [Journal Article] Left-right olfactory asymmetry results from antagonistic functions of voltage-activated calcium channels and the Raw repeat protein OLRN-1 in C. elegans. (2007)
  • Author(s): Bauer Huang, SL., Saheki, Y., Vanhoven, MK., Torayama, I., Ishihara, T., Katsura, I, van der Linden, A., Sengupta, P., Bargmann, CI
  • Journal Title: Neural Development 2 Pages: 24
  • Peer Reviewed
• [Journal Article] A Genome-wide Survey and Systematic RNAi-based Characterization of Helicase-like Genes in Caenorhabditis elegans (2007)
  • Author(s): Eki, T., Ishihara, T., Katsura, I., Hanaoka, F.
  • Journal Title: DNA Research 14 Pages: 183-199
  • Peer Reviewed
• [Journal Article] Caenorhabditis elegans integrates the signals of butanone and food to enhance chemotaxis to butanone. (2007)
  • Author(s): Torayama, I., Ishihara, T., Katsura
  • Journal Title: I. Journal of Neuroscience 27 Pages: 741-750
  • Peer Reviewed
• [Journal Article] IFT-81 and IFT74 are required for intraflagellar transport in C. elegans. (2007)
  • Author(s): Kobayashi, T., Gengyo-Ando, K., Ishihara, T., Katsura, I., Mitani
  • Journal Title: S. Genes To Cells 12 Pages: 593-602
  • Peer Reviewed
• [Presentation] MOLECULAR ANALYSES OF THE INTEGRATION OF TWO SENSORY SIGNALS IN C. (2010)
  • Author(s): Shinkai, Y., Ishihara, T.
  • Organizer: ELEGANS 2010 Cold Spring Harbor Laboratory Meeting on Neuronal Circuits
  • Place of Presentation: Cold Spring Harbor U.S.A.
  • Year and Date: 2010-03-10
• [Presentation] Chemotactic control by a germline signal in C. elegans (2009)
  • Author(s): Fujiwara, M., Sato, N., Maruyama, S., Akamine, T., Ishihara, T.
  • Organizer: 17th International C. elegans Meeting
  • Place of Presentation: Los Angeles U.S.A.
  • Year and Date: 20090624-20090628
• [Presentation] Molecular analysis of the integration of two sensory signals in C. elegans (2009)
  • Author(s): Shinkai, Y., Ishihara, T.
  • Organizer: 17th International C. elegans Meeting
  • Place of Presentation: Los Angeles U.S.A.
  • Year and Date: 20090624-20090628
• [Presentation] 線虫における感覚情報処理の分子機構Molecular mechanisms of sensory processing in C. elegans (2008)
  • Author(s): 石原健
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 20080709-20080711
• [Presentation] Molecular Mechanisms Of The Integration Of Two Sensory Signals In C. elegans. (2008)
  • Author(s): Shinkai, Y., Tsunozaki, M., Bargmann, C., Ishihara, T.
  • Organizer: NEWRONAL DEVELOPMENT, SYNAPTIC FUNCTION & BEHAVIOR C. elegans Topic Meeting #2
  • Place of Presentation: Madison, U.S.A.
  • Year and Date: 20080629-20080702
• [Presentation] Molecular mechanisms regulating the sensory integration process in C. elegans (2007)
  • Author(s): 新海陽一, 石原健
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 20071211-20071215
• [Presentation] Genetic analyses of mechanism for memory erasing in Caenorhaditis elegans. (2007)
  • Author(s): Inoue, A., Ishihara, T.
  • Organizer: 16 th International C. elegans meeting
  • Place of Presentation: Los Angeles, U.S.A.
  • Year and Date: 20070627-20070701
• [Presentation] Genetic analysis of the sensory integration process in the neuronal circuit. (2007)
  • Author(s): Shinkai, Y., Ishihara, T.
  • Organizer: 16 th International C. elegans meeting
  • Place of Presentation: Los Angeles, U.S.A.
  • Year and Date: 20070627-20070701
• [Book] 神経の分化,回路形成,機能発現 (2007)
  • Author(s): 石原健
  • Total Pages: 587-593
  • Publisher: 共立出版
• [Book] 脳機能研究の新展開 (2006)
  • Author(s): 石原健
  • Total Pages: 130-136
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.biology.kyushu-u.ac.jp/~bunsiide/