Mechanism of Biogenesis and Catalytic Function of Peptidyl Built-in Quinone Cofactors

2009 Fiscal Year Final Research Report

• Project/Area Number: 18370043
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: TANIZAWA Katsuyuki Osaka University, 産業科学研究所, 教授 (20133134)
• Co-Investigator(Kenkyū-buntansha): OKAJIMA Toshihide 大阪大学, 産業科学研究所, 准教授 (10247968)
• Project Period (FY): 2006 – 2009
• Keywords: 銅アミン酸化酵素 / トパキノン / ビルトイン型キノン補酵素 / キノヘムプロテイン / 分子内チオエーテル架橋 / 翻訳後修飾 / X線結晶解析 / 酵素触媒機構

Research Abstract

Peptidyl built-in cofactors are produced by posttranslational modification of the cognate enzyme proteins, unlike ordinary cofactors which are mostly bio-synthesized from water soluble vitamins. In this study, we focused on two built-in quinone cofactor-containing enzymes, copper amine oxidase and quinohemoprotein amine dehydrogenase (QHNDH) that contain topa quinone (TPQ) and cysteine tryptophyl quinone (CTQ), respectively. For the reaction mechanism of copper amine oxidase, it has been demonstrated that the former reductive half-reaction is accompanied with the conformational change of TPQ. On the other hand, two proteins encoded close to the QHNDH genes, an [Fe-S]-cluster and S-adenosylmethionine-binding protein and a subtilisin-like protease, have been found to play essential roles for QHNDH biogenesis.

Research Products

• [Journal Article] 分子内架橋構造をもちトリプトファン残基に由来するキノン補酵素の生合成機構 (2009)
  • Author(s): 岡島俊英, 谷澤克行
  • Journal Title: 化学と生物 47 Pages: 522-524
• [Journal Article] Further Insight into the Mechanism of Stereoselective Proton Abstraction by Bacterial Copper Amine Oxidase. (2008)
  • Author(s): Taki, M., Murakawa, T., Nakamoto, T., Uchida, M., Hayashi, H., Tanizawa, K., Yamamoto, Y., Okajima, T.
  • Journal Title: Biochemistry 47 Pages: 7726-7733
  • Peer Reviewed
• [Journal Article] Trapping of a Dopaquinone Intermediate in the TPQ Cofactor Biogenesis in a Copper-containing Amine Oxidase from Arthrobacter globiformis. (2007)
  • Author(s): Moore, R.H., Spies, M.A., Culpepper, M.B., Murakawa, T., Hirota, S., Okajima, T., Tanizawa K., Mure, M.
  • Journal Title: J. Am. Chem. Soc. 129 Pages: 11524-11534
  • Peer Reviewed
• [Journal Article] nvolvement of a Putative [Fe-S]-Cluster-Binding Protein in the Biogenesis of Quinohemoprotein Amine Dehydrogenase. (2006)
  • Author(s): Ono, K., Okajima, T., Tani, M., Kuroda, S., Sun, D., Davidson, V.L., Tanizawa, K.
  • Journal Title: J. Biol. Chem. 281 Pages: 13672-13684
  • Peer Reviewed
• [Journal Article] Kinetic and Structural Studies on the Catalytic Role of the Aspartic Acid Residue Conserved in Copper Amine Oxidase. (2006)
  • Author(s): Chiu, Y.-C., Okajima, T., Murakawa, T., Uchida, M., Taki, M., Hirota, S., Kim, M., Hayashi, H., Yamamoto, Y., Tanizawa, K.
  • Journal Title: Biochemistry 45 Pages: 4105-4120
  • Peer Reviewed
• [Journal Article] Quantum Mechanical Hydrogen Tunneling in Bacterial Copper Amine Oxidase Reaction. Biochem. (2006)
  • Author(s): Murakawa, T., Okajima, T., Kuroda, S., Nakamoto, T., Taki, M., Yamamoto, Y., Hayashi, H., Tanizawa, K.
  • Journal Title: Biophys. Res. Commun. 342 Pages: 414-423
  • Peer Reviewed
• [Journal Article] 新しいビルトイン型キノン補酵素CTQと分子内チオエーテル架橋構造-その特異な構造形成機構の解明に向けて-. (2006)
  • Author(s): 谷澤克行
  • Journal Title: 酵素工学ニュース 56 Pages: 5-9
• [Journal Article] X線結晶解析と反応速度論的解析に基づく銅アミン酸化酵素の触媒機構. (2006)
  • Author(s): 岡島俊英, 谷澤克行
  • Journal Title: 日本応用酵素協会誌 41 Pages: 1-7
• [Presentation] Acid-Base Chemistry in the Reductive Half-Reaction of Copper Amine Oxidase from Arthrobacter globiformis (2010)
  • Author(s): Murakawa, T., Hayashi, H., Okajima, T., Tanizawa, K.
  • Organizer: Gordon Research Conference on Protein Cofactors, Radicals and Quinones
  • Place of Presentation: Ventura, CA, USA
  • Year and Date: 20101024-20101029
• [Presentation] X-ray Crystallographic Evidence for Conformational Change of Topaquinone in the Reductive Half-reaction of Copper Amine Oxidase from Arthrobacter globiformis, Gordon Research Conference on Protein Cofactors (2010)
  • Author(s): Hamaguchi, A., Okajima, T., Nakai, T. Tanizawa, K., Murakawa, T., Hayashi, H.
  • Organizer: Radicals and Quinones
  • Place of Presentation: Ventura, CA, USA
  • Year and Date: 20100124-20101029
• [Presentation] 銅含有アミン酸化酵素のトパキノン補酵素生成におけるコンセンサス配列中のアスパラギン残基の役割 (2008)
  • Author(s): 元山暁仁、中西将太、黒田俊一、岡島俊英、谷澤克行
  • Organizer: 第31回日本分子生物学会年会/第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 神戸国際会議場・神戸国際展示場(兵庫県)
  • Year and Date: 20081209-20081212
• [Presentation] Involvement of an Iron Sulfur Protein and a Subtilisin-like Protease in the Biogenesis of Quinohemoprotein Amine Dehydrogenase (2008)
  • Author(s): Okajima, T., Ono, K., Tanizawa, K.
  • Organizer: Japan-Holland Joint Seminar on New Aspects in Enzyme Science and Biotechnology
  • Place of Presentation: 九州大学西新プラザホール(福岡県)
  • Year and Date: 20080928-20080930
• [Presentation] Conformational Flexibility of the TPQ Cofactor in Bacterial Copper Amine Oxidase, Gordon Research Conference on Protein Cofactors (2008)
  • Author(s): Okajima, T., Nakanishi, S., Murakawa, T., Hayashi, H., Tanizawa, K.
  • Organizer: Radicals and Quinones
  • Place of Presentation: Ventura, CA, USA
  • Year and Date: 20080120-20080125
• [Book] Cofactors of Amine Oxidases: Copper Ion and Its Substitution and the 2, 4, 5-Trihydoxylphenylalanine Quinone (Chapter 3). In'Copper Amine Oxidases: Structures, Catalytic Mechanisms, and Role in Pathophysiology'(Taylor & Francis Group) (2009)
  • Author(s): Suzuki, S., Okajima, T., Tanizawa, K., Mure, M.
  • Total Pages: 19-38
  • Publisher: CRC Press
• [Book] Mechanism of TPQ Biogenesis in Prokaryotic Copper Amine Oxidase (Chapter 8). In'Copper Amine Oxidases: Structures, Catalytic Mechanisms, and Role in Pathophysiology'(Taylor & Francis Group) (2009)
  • Author(s): Okajima, T., Tanizawa, K.
  • Total Pages: 103-118
  • Publisher: CRC Press
• [Remarks]
  • URL: http://www.sanken.osaka-u.ac.jp/labs/smb/project.html