Elucidation of regulatory mechanisms of vanilloid receptor on pain signaling

2008 Fiscal Year Self-evaluation Report

• Project/Area Number: 18380171
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Tottori University (2008-2009); Hokkaido University (2006-2007)
• Principal Investigator: OHTA Toshio Hokkaido University, 大学院・獣医学研究科, 准教授 (20176895)
• Project Period (FY): 2006 – 2009
• Keywords: TRPV1 / capsaicin / intracellular Ca / pain

Research Abstract

関連受容体であるバニロイド受容体遺伝子をブタ、ラット、マウスからクローニングし、異所性発現させ、機能解析する。バニロイド受容体活性化を制御するメカニズムを明らかにし、本受容体に作用する薬物(鎮痛薬の候補物質)を検索する。

Research Products

• [Journal Article] Lipopolysaccharides enhance the action of bradykinin in enteric neurons via secretion of interleukin-1 β from enteric glial cells (2009)
  • Author(s): Murakami M, Ohta T, Ito S.
  • Journal Title: J. Neurosci. Res.
• [Journal Article] Involvement of transient receptor potential vanilloid subtype 1 (TRPV1) in analgesic action of methylsalicylate (2009)
  • Author(s): Ohta T, Imagawa T, Ito S.
  • Journal Title: Mol. Pharmacol 75 Pages: 307-317
• [Journal Article] Novel gating and sensitizing mechanism of capsaicin receptor (TRPV1) : Tonic inhibitory regulation of extracellular sodium through the external protonation sites on TRPV1 (2008)
  • Author(s): Ohta T. Imagawa T, Ito S.
  • Journal Title: J. Biol. Chem. 283 Pages: 9377-9387
• [Journal Article] Voltage-gated channel properties of epithelial cells in porcine vorneronasal organ (2008)
  • Author(s): Ishida T, Takei R, Gautam SH, Otsuguro K, Ohta T, Ito S, Habara Y, Saito, T.
  • Journal Title: Neurosci. Lett. 441 Pages: 277-281
• [Journal Article] Why does carbon dioxide produce analgesia? (2008)
  • Author(s): Otsuguro K, Yasutake S, Yamaji Y, Ban M, Ohta T, Ito S.
  • Journal Title: Alternatives to Animal Testing and Experimentation 14 Pages: 101-106
• [Journal Article] Interleukin lbeta enhances the action of bradykinin in rat myenteric neurons through upregulation of glial B1 receptor expression (2008)
  • Author(s): Murakami M, Ohta T. Ito S.
  • Journal Title: Neuroscience 151 Pages: 222-231
• [Journal Article] Novel agonistic action of mustard oil on recombinant and endogenous porcine Transient Receptor Potential VI (pTRPV1) channels (2007)
  • Author(s): Ohta T, Imagawa T, Ito S.
  • Journal Title: Biochem. Pharmacol 73 Pages: 646-656
• [Journal Article] Involvement of prostaglandin E_2 derived from enteric glial cells in the action of bradykinin in cultured rat myenteric neurons (2007)
  • Author(s): Murakami M, Ohta T, Otsuguro K, Ito S.
  • Journal Title: Neuroscience 145 Pages: 642-653
• [Journal Article] Potentiation of transient receptor potential V1 functions by the activation of metabotropic 5-hydroxytryptamine receptors in rat primary sensory neurons (2006)
  • Author(s): Ohta T, Ikemi Y, Murakami M, Imagawa T, Otsuguro K, Ito S.
  • Journal Title: J. Physiol 576 Pages: 809-822
• [Journal Article] Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines (2006)
  • Author(s): Takahashi B, Inanami O, Ohta T, Matsuda A, Kuwabara M.
  • Journal Title: Leuk. Res. 31 Pages: 1555-1561
• [Journal Article] Involvement of adenosine in depression of synaptic transmission during hypercapnic acidosis in isolated spinal cord of neonatal rats (2006)
  • Author(s): Otsuguro K, Yamaji Y, Ban M, Ohta T, Ito S.
  • Journal Title: J. Physiol 574 Pages: 835-847
• [Journal Article] Zinc modulates primary afferent fiber-evoked responses of ventral roots in neonatal rat spinal cord in vitro (2006)
  • Author(s): Otsuguro K, Ohta T, Ito S.
  • Journal Title: Neuroscience 138 Pages: 281-191
• [Presentation] TRPVlチャネルの細胞外ナトリウムイオンによる活性調節 (2008)
  • Author(s): 太田利男、今川敏明、伊藤茂男
  • Organizer: 第4回TRPチャネル研究会
  • Place of Presentation: 生理学研究所、岡崎カンファレンスセンター
  • Year and Date: 20080605-06
• [Presentation] AIIylisothiocyanateによるTRPVIチャンネルに対する活性化作用 (2007)
  • Author(s): 太田利男、鳥居優人、今川敏明、伊藤茂男
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 酪農大
  • Year and Date: 2007-09-03
• [Presentation] 5-Hydroxytryptamine potentiates transient receptor potential V1 functions in rat primary sensory neurons (2006)
  • Author(s): Toshio Ohta, Ikemi Yuki, Murakami Matsuka, Otsuguro Kenichi, Ito Shigeo
  • Organizer: J. Pharmacol. Sci. 101, Supple 1, p134 (Sertonin Club, 6^<th> IUPHAR Satellite Meeting on Serotonin
  • Place of Presentation: Sapporo
  • Year and Date: 20060727-30
• [Presentation] 642.4 Metabotolopic 5-hydroxytryptamine receptors are involved in potentiation of transient receptor potential V1 function in rat primary sensory neurons (2006)
  • Author(s): Ohta Toshio, Ikemi Yuki, Murakami Matsukam, Otsuguro Kenichi, Ito Shigeo
  • Organizer: Neuroscience2006
  • Place of Presentation: Atranta, USA
  • Year and Date: 2006-10-17
• [Presentation] AllylisothiocyaneteによるTransient ReceptorPotentialV1(TRPV1)チャンネルに対する刺激作用
  • Author(s): 太田利男、今川敏明、伊藤茂男
  • Organizer: 第80回日本薬理学会年会(Action of allylisothiocyanate on recombinant and endogenous porcine transient receptor potential V1 (pTRPV1) channels. J. Pyarmacol. Sci. 103, Supple 1.256p)
  • Place of Presentation: 名古屋
• [Presentation] 神経因性痙痛発現におけるTransient Recept or Potential V1(TRPV1)の関与について-TRPV1ノックアウトマウスを用いた解析-
  • Author(s): 太田利男、小松竜一、乙黒兼一、伊藤茂男
  • Organizer: 第142回日本獣医学会学術集会講演要旨集174p日本獣医学会
  • Place of Presentation: 山口大
• [Presentation] ブタTRPV1遺伝子クローニングと発現機能解析-w内因性TRPV1受容体反応との比較-(P2N-34)
  • Author(s): 太田利男、小松竜一、今川敏明、乙黒兼一、伊藤茂男
  • Organizer: Molecular cloning, functional characterization of the porcine TRPV1 (pTRPV1) and pharmacological comparison with endogenous pTRPV1. J. Pharm. Sci. (100Suppl 1.238P)