2009 Fiscal Year Final Research Report
Regulatory mechanisms of vanilloid receptor on pain signaling
| Project/Area Number |
18380171
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Tottori University (2008-2009)
Hokkaido University (2006-2007) |
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Principal Investigator |
OHTA Toshio Tottori University, 農学部, 教授 (20176895)
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| Co-Investigator(Kenkyū-buntansha) |
IMAGAWA Toshiaki 北海道大学, 大学院・先端生命科学研究院, 准教授 (20142177)
INANAMI Osamu 北海道大学, 大学院・獣医学研究科, 教授 (10193559)
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| Project Period (FY) |
2006 – 2009
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| Keywords | TRPV1 / capsaicin / ion channel / intracellular Ca / pain |
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| Research Abstract |
We cloned cDNA encording vanilloid receptor (TRPV1) from porcine and human spinal cord cDNA library. We established in vitro assay system for monitoring vanilloid receptor activities. Heterologous expressed TRPV1 receptors are activated by not only capsaicin, heat and protons, but also regulated by extracellular sodium ions. Hyperalgesia induced by inflammatory mediators, such as serotonin and histamine is due to a hyper-activation of TRPV1 using heterologous expression system and cultured sensory neurons. We found that mustard oil and methylsalicylate act as TRPV1 agonist and the latter produces marked desensitization of TRPV1 resulting in anti-nociception for capsaicin-induced pain. These data suggest that TRPV1 is important target molecule especially for inflammation-induced hyperalgesia.
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