2007 Fiscal Year Final Research Report Summary
Mechanism and role of phagocytosis of dying cells and microbes
| Project/Area Number |
18570123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
SHIRATSUCHI Akiko Kanazawa University, Graduate School of Medical Science, Associate Professor (90303297)
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| Project Period (FY) |
2006 – 2007
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| Keywords | phagocytosis / apoptosis / signal transduction / microbes / spermatogenesis / innate immunity / Toll-like receptor / influenza virus |
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| Research Abstract |
1) Mechanism and role of SR-BI-mediated apoptotic spermatogenic cells by Sertoli cells in the testis. After phagocytosis of apoptotic spermatogenic cells, testicular somatic Sertoli cells produced unknown factor(s) by which spermatogenic cells could differentiate into spermatozoa. To identify the factor, we selected one candidate protein by proteome analysis of Sertoli cells and identified its primary structure. We found that the expression of the factor was stimulated in transcriptional level during phagocytosis. We have been generating knockout mice of the factor, and we are going to examine the role of the factor on spermatogenesis in mice.
2) Role of TLR-JNK pathway on bacterial survival in macrophages Staphylococcus aureus induced JNK phosphorylation in mouse peritoneal macrophages depending on toll-like receptor TLR2. This activation of INK pathway caused inhibition of superoxide production in the macrophages, and this allowed engulfed S. aureus to survive in the macrophages.
3) Role of phagocytosis of influenza virus-infected cells by macrophages Influenza virus-infected cells produced macrophage-activating factor that enhance macrophage phagocytosis of virus-infected cells depending on both virus infection and apoptosis. This factor would be heat-labile material since it lost the activity after heat-treatment.
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[Journal Article] Bridging effect of recombinant human mannose-binding lectin in macrophage phagocytosis of Eschericha coli2008
Author(s)
Shiratsuchi, A., Watanabe, I., Ju, J.,-S., Lee, B.,-L., Nakanishi, Y
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Evidence for phagocytosis of influenza virus-infected, apoptotic cells by neutrophils nad macrophages in mice.2007
Author(s)
Hashimoto, Y., Moki, T., Takizawa, T., Shiratsuchi, A., and Nakanishi, Y.
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Journal Title
J. Immunol. 178
Pages: 2448-2457
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Perturbation of spermatogenesis by androgen antagonists directly injected into seminiferous tubules of live mice.2007
Author(s)
Nagaosa, K., Kishimoto, A., Kizu, R., Nakagawa, A. Shiratsuchi, A., and Nakanishi, Y.
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Journal Title
Reproduction 133
Pages: 21-27
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Evidence for phagocytosis of influe nza virus-infected, apoptotic cells by neutrophils and macrophages in mice2007
Author(s)
Hashimoto, Y., Moki, T.,Takizawa,T., Shiratsuchi, A., Nakanishi, Y
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Journal Title
J. Immunol 078
Pages: 2448-2457
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Perturbation of sper matogenesis by androgen antagonists directly injected i nto seminiferous tubules of live mice2007
Author(s)
Nagaosa, K., Kishimoto, A., Kin, R., Nakagawa, A., Shiratsuchi, A., Nakanishi, Y
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Journal Title
Reproduction 133
Pages: 21-27
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 精細管内の細菌に対する免疫応答の解析2007
Author(s)
永長一茂, 中嶋千恵, 長田洋一, 岸本淳司, 白土明子, 中西義信
Organizer
第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
Place of Presentation
横浜
Year and Date
2007-12-14
Description
「研究成果報告書概要(和文)」より
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[Presentation] Isolation of an endoplasminc reticulum protein as a candidate ligand for Draper, a Drosophila phagocytosis receptor2007
Author(s)
Kuraishi,T., Yamamoto, N., Shiratsuchi, A., Yoshinobu, Nakanishi, et. al.
Organizer
8th Japan Drosophila Meeting
Place of Presentation
Awaji, Japan
Year and Date
2007-07-02
Description
「研究成果報告書概要(欧文)」より
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