2007 Fiscal Year Final Research Report Summary
Studies on the Identification and Differentiation of Tissue Stem Cells in Exocrine Organs by 3-D Immunohistochemistry
| Project/Area Number |
18590193
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kitasato University |
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Principal Investigator |
YAMASHINA Shohei Kitasato University, School of Medicine, Emeritus Professor (90013987)
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| Co-Investigator(Kenkyū-buntansha) |
KADOYA Yuichi Kitasato Univ., School of Medicine, Assistant Professor (10185887)
TAMAKI Hideaki Kitasato Univ., School of Medicine, Assistant Professor (30155246)
HAYASHI Keiko Kitasato Univ., School of Medicine, Research Associate (30240211)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Embryology / Histogenesis / Cellular Differentiation / 3-D reconstruction / Secretory Organ / Tissue Stem Cell / Intercalated Duct Cell / Pancreas |
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| Research Abstract |
1. Identification of tissue stem cells in the salivary gland In the rat parotid gland after repeated injection by isoproterenol, differentiation potential was studied by means of multiple immunohistochemistry. As a result, followings were concluded. (1) Terminal cells in the intercalated duct differentiate toward acinar cells at 3rd days after IPR treatment. (2) Differentiation was not demonstrated in cells in other portion of intercalated duct by the experimental system.
2. Differntiation potential of intercalated duct cells after the partial pancreatectomy During the course of regeneration after the partial pancreatectomy, 3-D reconstruction was performed from serial section after the treatment of multiple immunohistochemistry of cytokeratin 20 and insulin. As a result, it was found that cells at the terminal portion of intercalated duct differentiated toward acinar cells and cells at the branching portion of intercalated duct differentiated into endocrine cells.
3. Determination of endocrine stem cells in the developing rat pancreas Development of pancreatic islets was investigated by means of 3-D reconstruction treated by multiple immunohistochemistry. Glucagon positive cells were found to appear in the various portion of tubulo-acinar structure during 15 to 17 days in embryonic days, and plural endocrine cells gathered to form a single islet, indicating that the endocrine cells in a islet were not a clone but multiple origin.
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Research Products
(10 results)
