2007 Fiscal Year Final Research Report Summary
Molecular analysis of tissue-specific imprinting
| Project/Area Number |
18590273
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Nagasaki University |
|---|
Principal Investigator |
KISHINO Tatsuya Nagasaki University, Center for Frontier Life Sciences, Division of Functional Genomics, Associate Professor (70315232)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | imprinting / epigenetics / DNA methvyation / histone modifications / neuron |
|---|
| Research Abstract |
Most imprinted genes display parental-origin-specific gene expression in all tissue where they are transcribed, however some genes are imprinted in a tissue-specific manner. Genes that show brain-specific imprinting or brain-specific lack of imprinting present a unique opportunity to study the process of imprinting during tissue differentiation. In this project, I analyzed brain cell lineage-specific imprinting using a primary brain cell culture system and discussed imprinting-associated epigenetic modifications in neurons. I focused on the imprinted genes, Grb10, and Snrpn-Ube3a, which. show neuron-specific imprinting and analyzed DNA methylation, histone modifications and antisense RNA.
|
