2007 Fiscal Year Final Research Report Summary
An analysis of the role of protein kinase PKN3 in the progress of cancer and a development of new anticancer agent
| Project/Area Number |
18590294
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kobe University |
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Principal Investigator |
MUKAI Hideyuki Kobe University, Biosignal Research Center, Associate Professor (80252758)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Rho / small GTPase / PKN3 / protein kinase / cancer / oncogenesis / knockout mice |
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| Research Abstract |
We prepared protein kinase PKN3 knockout mice to analyze the function of PKN3 in vivo. PKN3 knockout mice did not show any significant macroscopic morphological abnormalities and problems in reproductive potential and the feeding behavior. We isolated embryonic fibroblasts from PKN3 knockout mice and examined the wound healing activity and RasV12 induced focus forming activity, comparing with the wild type mice. And we crossed PKN3 knockout mice with pten conditional knockout mice which are susceptible to malignant neoplasms, and examined the effect of PKN3 on the tumorigenesis and metastasis. In order to prepare a specific inhibitor for PKN3, we prepared various deletion mutants and point mutants of PKN3 and analyzed the activation mechanism of PKN3 by checking the protein phosphorylation activity of the enzyme. We also checked the effect of PDK1 on the stability and the activation mechanism of PKN3.
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[Journal Article] Fragmentation of Protein Kinase N(PKN)in the Hydrocephalic Rat Brain2007
Author(s)
Okii, N, Amano, T, Seki, T, Matsubayashi, H, Mukai, H, Ono, Y, Kurisu, K, Sakai, N
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Journal Title
Acta Histochem Cytochem 40
Pages: 113-21
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Calcineurin-mediated dephosphorylation of c-Jun Ser-243 is required for c-Jun protein stability and cell transformation
Author(s)
Huang, CC, Wang, JM, Kikkawa, U, Mukai, H, Shen, MR, Morita, I, Chen, BK, Chang, WC
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Journal Title
Oncogene(2007) (in press)
Description
「研究成果報告書概要(欧文)」より
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[Presentation] An identification of the substrate recognition motif and a development of inhibitory peptide for PKN2007
Author(s)
Shiga, K, Ueki, K, Hutti, J, Maeda, T, Turk, B, Ono, Y, Mukai, H
Organizer
BMB2007
Place of Presentation
Pacifico Yokohama
Year and Date
2007-12-11
Description
「研究成果報告書概要(欧文)」より
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[Book] ゲノム科学2008
Author(s)
向井秀幸
Publisher
京都廣川書店(in press)
Description
「研究成果報告書概要(和文)」より
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