2009 Fiscal Year Final Research Report
Gene Transfer of Human Extracellular Superoxide Dismutase Prevents Monocrotaline-Induced Pulmonary Hypertension in Rats
| Project/Area Number |
18590827
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
TASAKI Hiromi University of Occupational and Environmental Health, Japan, 医学部, 非常勤医師 (60216950)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Kazuhito 産業医科大学, 医学部, 助教 (00341496)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUTSUI Masato 琉球大学, 医学部, 教授 (70309962)
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| Project Period (FY) |
2006 – 2009
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| Keywords | 肺高血圧症 / スーパーオキシドジスムターゼ / モノクロタリン / 遺伝子治療 / ECSOD |
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| Research Abstract |
In the present study, we examined whether intratracheal gene transfer of human extracellular superoxide dismutase (ECSOD) could ameliorate monocrotaline (MCT)-induced PAH in rats. MCT-injected rats were concomitantly intratracheally administered vehicle (MCT group), an adenovirus expressing β-galactosidase (Adβgal group), or human ECSOD (AdECSOD group). Twenty-eight days after the MCT injection, right ventricular systolic pressure and the weight ratio of the right ventricle to the left ventricle plus septum were significantly lower in the AdECSOD group. As the mechanism of these effects, 8-isoprostane in lung tissue was significantly reduced in the AdECSOD group. ECSOD overexpression to the lung ameliorated MCT-induced PAH in rats.
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