2007 Fiscal Year Final Research Report Summary
Free radical production from betaAmyloid oligomers and mechanism of neurotoxicity deduced from them
| Project/Area Number |
18591298
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Psychiatric science
|
|---|
| Research Institution | University of Miyazaki |
|---|
Principal Investigator |
HAYASHI Yoshihito University of Miyazaki, Faculty of Medicine, Assistant Professor (20295223)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ISHIDA Yasushi UNIVERSITY OF MIYAZAKI, Faculty of Medicine, Professor (20212897)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | beta amyloid / free radical / oligomer |
|---|
| Research Abstract |
It is well known that beta amyloid (Aβ) peptides aggregate and make senile plaques up in AD patients' brain. So far the causes of AD is thought to be due to the excessive production of Aβ and its toxicity against neuronal cells in the brain in the process of aggregation. One of the main toxicities that Aβ shows against neuronal cells is due to productions of free radicals. It is thought that the stage when free radicals are produced maximally by Aβ is the very early period and it is just when Aβ changes into oligomers such as dimmer, timer and tetramer. This time we make oligomers (dimmer, trimmer, tetramer) using photocross-linking methods and separate them individually by using size gelchoromatography. Then we measured how much these oligomers produce H2O2 using APR solution and how many % of cells survive in the circumstances with Aβ oligomers. The results were that the molecular size of oligomers goes up there is tendency that the production of H2O2 is going up within 24 hours but there are no apparent differences between four forms of Aβ oligomers. The survive rate of the cells after 48hours about 60% cell were survived and there were also no apparent differences between Aβ oligomers. Therefore it will be needed that we should do further experiment using more bigger size oligomers such as tetramer, hexamer and soon carefully.
|
