2007 Fiscal Year Final Research Report Summary
CANCER DIAGNOSIS BY SIMPLE SEQUENCE REPEAT IN THE INTRON THAT AFFECTS ALTERNATIVE APLICING OF C-MYCTRANSCRIPTIONALSUPPRESSOR,FIR
| Project/Area Number |
18591406
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Chiba University |
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Principal Investigator |
SAKATA Haruhito Chiba University, CHIBA UNIVERSITY HOSPITAL, FRONTIER SURGERY, ASSISTANT PROFESSOR (20375687)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUSHITA Kazuyuki CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICIE, EMERGENCYAND CRITICAL CARE MEDICINE, ASSISTANT PROFESSOR (90344994)
SHIMADA Hideaki CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICIE, FRONTIER SURGERY, ASSOCIATE PROFESSOR (20292691)
SUNAGA Masahiko CHIBA UNIVERSITY, CHIBA UNIVERSITY HOSPITAL, MOLECULAR DIAGNOSIS, ASSISTANT PROFESSOR (10361437)
TOMONAGA Takeshi CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICIE, MOLECULAR DIAGNOSIS, ASSOCIATE PROFESSOR (80227644)
NISHIMORI Takanori CHIBA UNIVERSITY, CHIBA UNIVERSITY HOSPITAL, FRONTIER SURGERY, ASSISTANT PROFESSOR (30401003)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Alternative splicing / simple sequence repeat / intron / transcriptional factor / cancer diagnosis |
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| Research Abstract |
Detection of cancer cells in the peripheral blood (PB) is pivotal for prediction of micro-metastasis, recurrence probability and/or outcome of the patients. Far UpStream Element (FUSE)-binding protein-interacting repressor, FIR, is a transcriptional repressor of c-myc gene. FIRΔexon2, exon 2-spliced variant of FIR, which lacks c-myc repression activity, was frequently discovered in human primary colorectal cancers but not in corresponding non-cancer epithelium, indicating its cancer-related expression. In this study, thus the expression level of FIRΔexon2 mRNA is examined in PB of 30 colorectal cancer patients and compared to those of 17 healthy volunteers by real-time polymerase chain reactions. As expected, FIRΔexon2 mRNA expression in PB of cancer patients significantly activated than those of healthy volunteers. Additionally, FIRΔexon2 as well as FIR mRNA is detected in PB even in the early stage (Dukes A stage) of colorectal cancer patients. The area under the receiving operating characteristic curve of FIRΔexon2 was greater than conventional carcinoembryonic antigen or carbohydrate antigen 19-9. Together, FIRΔexon2 mRNA in PB is a candidate of novel screening marker for colorectal cancer detection.
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