2007 Fiscal Year Final Research Report Summary
Analysis of the antitumor immunity activity of α-GalCer according to each organ against the human tumor cell line
| Project/Area Number |
18591443
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
FUJI Nobuaki Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Assistant Professor (90332949)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Tsuyoshi Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Assistant Professor (40420707)
YAMAGISHI Hisakazu Kyoto Prefectural University of Medicine, President (40128723)
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| Project Period (FY) |
2006 – 2007
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| Keywords | α-GalCer |
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| Research Abstract |
We presented mechanism of the antitumor effect of a-GalCer using a murine spontaneous hepatic metastases model. Therefore this purpose was to investigate antitumor effect of a-GalCer against the human tumor cell line in vitro. At first lymphocytes were divided from human peripheral blood or liver tissue, and a-GalCer was added in them in vitro. Cellular cytotoxicity for the human tumor cell line and a production of cytokine having the anti-tumor activity were measured, and effectors cells were analyzed subsequently. Furthermore, we schemed to investigate whether anti-tumor activity of a-GalCer had organ specificity in a human. The reason was because a-GalCer administration could become a treatment for the metastasis lesion of the cancer patient if this antitumor effect had organ specificity.
A study of the antitumor effect of peripheral blood lymphocyte and hepatic lymphocyte: From the patient who underwent abdominal operation peripheral blood and hepatic tissue were obtained, and peripheral blood lymphocytes and hepatic lymphocytes were divided. Human tumor cell line and a-GalCer were added at various kinds of concentration for each lymphocyte, and mixed culture was performed in vitro, and cytotoxic activity by the Cr release examination was measured. However, results having reproducibility could not be obtained in peripheral blood lymphocyte and hepatic lymphocyte. Therefore a further experiment was needed to establish optimal concentration of a-GalCer. Quantity of production of IFN -y or IL-12 could not be evaluated continuously.
Effectors analysis of the above lymphocytes: Effectors analysis was started in each lymphocyte for a few examples. However, we did not result in an outcome having reproducibility similarly. A process and an effort to prepare as possible the background of the patient will be most important in future. A scheme and an effort to keep as possible the background of the patient may be most important in future.
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Research Products
(20 results)
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[Journal Article] Potassium oxonate, an enzyme inhibitor compounded in S-1, reduces the suppression of antitumor immunity induced by 5-fluorouracil.2006
Author(s)
Yamashita, T, Ueda, Y, Fuji, N, Itoh, T, yamagishi, H, et. al.
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Journal Title
Cancer Chemother Pharmacol 58 (2)
Pages: 183-188
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A new strategy using autologous dendritic cells and lymphokine-activated killer cells for cancer immunotherapy: efficient maturation of DCs by co-culture with LAK cells in vitro.2006
Author(s)
Yano, Y, Ueda, Y, Itoh, T, Fuji, N, Yamagishi, H, et. al.
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Journal Title
Oncol Rep 16 (1)
Pages: 147-152
Description
「研究成果報告書概要(欧文)」より
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