2007 Fiscal Year Final Research Report Summary
Clinical Application of Tissue Engineered Vascular Autograft using Autologous Bone Marrow Cells and Biodegradable Scaffolds
| Project/Area Number |
18591563
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
MATSUMURA Goki Tokyo Women's Medical University, School of Medicine, Assistant Professor (20297469)
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| Co-Investigator(Kenkyū-buntansha) |
KUROSAWA Hiromi Tokyo Women's Medical University, School of Medicine, professor (50075511)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Tissue Engineering / Autologous Cells / Biodegradable Scaffolds / Surgery / Cardiovascular / Tissue / Bone Marrow Cells / 生力学試験 |
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| Research Abstract |
This study demonstrates the evaluation of the endothelial function and mechanical properties of tissue-engineered vascular autografts (TEVAs) constructed with autologous mononuclear-bone marrow cells (MN-BMCs) and a biodegradable scaffold using a canine inferior vena cava (IVC) model. MN-BMCs were obtained from a dog and seeded onto a biodegradable tubular scaffold consisting of polyglycolide fiber and poly(l-lactide-co-ε-caprolactone) sponge. This scaffold was implanted in the IVC of the same dog on the day of surgery. TEVAs were analyzed biochemically, biomechanically and histologically after implantation and observed for 1, 3, 6 and over 12 months.
Inner surface of TEVAs (8 mm in diameter and 2 cm in length) were covered with endothelial cells. Histologically, smooth muscle cells, collagen fibers and elastic fibers. which constitute the vessel wall increased gradually with time after implantation. And the specimens after a year after implantation revealed matured vessel-like-structure. The mechanical properties of TEVAs increased with time and showed similar strength as native tissue at a year after implantation, suggesting that the tissue regenerate with time in vivo to create a new "engineered-vessel". Furthermore, wall thicknesses of TEVAs at 6 months after implantation were similar to that of native tissue, and tolerated venous pressure well without any problems such as calcification. Histologically, inflammatory cells such as CD4 positive or MCP-1 positive cells in TEVAs decreased with time. These results indicate that TEVAs are biocompatible material with functional endothelial cells and biomechanical properties without unwanted side-effects.
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[Journal Article] Mid-term Clinical Results of Tissue-Engineered Vascular Autografts2007
Author(s)
Goki, Matsumura, Toshiharu, Shin'oka, Narutoshi, Hibino, Satoshi, Saito, Takahiko, Sakamoto, Yuki, Ichihara, Kyoko, Hobo, Shin'ka, Miyamoto, Hiromi, Kurosawa
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Journal Title
The Japanese Society for Cardio-vascular Surgery 36(6)
Pages: 309-314
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Evaluation of Tissue Engineered Vascular Autografts2006
Author(s)
Goki, Matsumura, Yoko, Ishihara, Sachiko, M., Tomita, Yoshito, Ikada, Shojiro, Matsuda, Hiromi, Kurosawa, Toshiharu, Shin'oka
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Journal Title
Tissue Engineering 12(11)
Pages: 3075-3083
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Midterm clinical result of tissue-engineered vascular autografts seeded with autologous bone marrow cells2005
Author(s)
Shin'oka, T., Matsumura, G., Hibino, N., Naito, Y., Watanabe, M., Konuma, T., Sakamoto, T., Nagatsu, M., Kurosawa, H
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Journal Title
J Thorac Cardiovasc Surg 129, 6
Pages: 1330
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The tissue-engineered vascular graft using bone marrow without culture2005
Author(s)
Hibino, N., Shin'oka, T., Matsumura, G., Ikada, Y., Kurosawa, H
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Journal Title
J Thorac Cardiovasc Surg 129, 5
Pages: 1064
Description
「研究成果報告書概要(欧文)」より
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[Presentation] A Novel Therapy Using Tissue Engineering Vascular Autografts2007
Author(s)
Goki, Matsumura, Toshiharu, Shin'oka, Kazuaki, Ishihara, Takahiko, Sakamoto, Yusuke, Iwata, Noboru, Yamamoto, Narutoshi, Hibino, Yuki, Ichihara, Shin'ka, Miyamoto, Kyoko, Hobo, Hiromi, Kurosawa, Hiromi, Kurosawa
Organizer
Japanese Society of Pediatric Cardiology and Cardiac Surgery
Place of Presentation
Tokyo
Year and Date
2007-07-15
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Mid-term Clinical Results of Tissue-Engineered Vascular Autografts2007
Author(s)
Goki, Matsumura, Toshiharu, Shin'oka, Narutoshi, Hibino, Satoshi, Saito, Takahiko, Sakamoto, Yuki, Ichihara, Kyoko, Hobo, Shin'ka, Miyamoto, Hiromi, Kurosawa
Organizer
The Japanese Society for Cardio- vascular Surgery
Place of Presentation
Tokyo
Year and Date
2007-02-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Time Coarse Evaluations of Tissue Engineered Grafts2000
Author(s)
Goki, Matsumura, Toshiharu, Shin'oka, Satoshi, Saito, Takahiko, Sakamoto, Yoshimichi, Kosaka, Yuki, Ichihara, Shin'ka, Miyamoto, Kyoko, Hobo, Hiromi, Kurosawa
Organizer
The Japanese Society for Cardio-vascular Surgery
Place of Presentation
Morioka
Year and Date
2000-04-14
Description
「研究成果報告書概要(欧文)」より
