Study on role of motor proteins in intracellular transport of pituitary hormone

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18591608
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Teikyo University
• Principal Investigator: MATSUNO Akira Teikyo University, School of Medicine, Professor (00242058)
• Co-Investigator(Kenkyū-buntansha): NAKAGOMI Tadayoshi Teikyo University, School of Medicine, Professor (90198052) ; FUJIMAKI Takamitsu Teikyo University, School of Medicine, Associate Professor (80251255) ; TAKEKOSHI Susumu Tokai University, School of Medicine, Associate Professor (70216878) ; OSAMURA Yoshiyuki Tokai University, School of Medicine, Professor (10100992)
• Project Period (FY): 2006 – 2007
• Keywords: growth hormone / rab3B / motor protein / kinesin superfamily / intracellular transport / pituitary cell

Research Abstract

We focused on kinesin superfamily (KIF) among motor proteins related with the intracellular transport of pituitary hormone.The identification of KIF working synergistically with rab 3B, which is an important molecule in growth hormone (GH) transport, was carried out.We synthesized fusion protein of KIF and green fluorescent protein (GFP). EGFP-KIF and ECFP-Rab3B were co-transfected in GH3 cell and AtT-20 cell that express EYFP-GH.Moreover, the suppression of KIF using siRNA was examined.We confirmed the suppression of KIF led to the inhibition of the secretion of GH.The results of these experiments were observed under confocal laser scanning microscopy.

Research Products

• [Journal Article] R-Ras Regulates Exocytosis by Rgl2/Rlf-mediated Activation of RalA on Endosomes (2007)
  • Author(s): Takaya A, et. al.
  • Journal Title: Mol Biol Cell 18 Pages: 1850-1860
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Growth hormone (2007)
  • Author(s): Matsuno A
  • Journal Title: J Neurosurg 106 Pages: 940-941
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] OctreotideによるGH産生下垂体腺腫細胞の縮小機構 (2007)
  • Author(s): 林 周兵, ら
  • Journal Title: ホルモンと臨床55巻 '07春季増刊号 内分泌病理学 最近の進歩2005/2006 55 Pages: 6-16
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] R-Ras Regulates Exocytosis by Rg12/Rlf-mediated Activation of RalA on Endosomes (2007)
  • Author(s): Takaya, A, Kamio, T, Masuda, M, Mochizuki, N, Sawa, H, Sato, M, Nagashima, K, Mizutani, A, Matsuno, A, Kiyokawa, E, Matsuda, M
  • Journal Title: Mol Biol Cel 18 Pages: 1850-1860
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Growt, h hormone (2007)
  • Author(s): Matsuno, A
  • Journal Title: J Neurosurg 106 Pages: 940-941
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] An insight of subcellular localization of pituitary hormone mRNA : observation under electron microscopy and confocal laser scanning microscopy (2007)
  • Author(s): A, Itoh J, Utsunomiya, H, Takekoshi, S, Osamura, RY
  • Journal Title: New Messenger RNA Research Communications, Nova Science Publishers, Inc. Matsuno Pages: 69-86
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Surgical and pathophysiological aspects of pituitary adenoma (2007)
  • Author(s): Matsuno A
  • Organizer: Samsung International Brain Tumor Symposium
  • Place of Presentation: Seoul,Korea
  • Year and Date: 2007-09-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Surgical and pathophysiological aspects of pituitary adenoma (2007)
  • Author(s): Matsuno, A
  • Organizer: Samsung International Brain Tumor Symposium
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2007-09-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] New Messenger RNA Research Communications, Nova Science Publishers, Inc. (2007)
  • Author(s): Matsuno A, et. al.
  • Total Pages: 69-86
  • Publisher: An insight of subcellular localization of pituitary hormone mRNA:observation under electron microscopy and confocal laser scanning microscopy
  • Description: 「研究成果報告書概要(和文)」より